E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents

Rohit Dixit, Prabhakar Raosaheb Patil.

Abstract
Background: Although roots of neem plant have established pharmacological activities such as analgesic, anti-inflammatory, antimicrobial, and anticarcinogenic, there are contradictory results as far as anticonvulsant activity is concerned. Hence, the present study was undertaken to explore the anticonvulsant activity of neem roots.

Objectives: The objectives of this study were to study the anticonvulsant activity of alcoholic extract of neem root.

Materials and Methods: In maximal electroshock method (maximal electroshock seizure), a total of 30 albino rats of either sex weighing 200–250 g were used and divided into six groups. The control group received 2 ml of distilled water orally daily for 15 days. The standard group received phenobarbitone 20 mg/kg i.p. The test groups received orally neem leaf extract at the dose of 200, 400, and 800 mg/kg for 15 days. Convulsions were produced by 150 mA current for 0.2 s on day 1, 3, 5, and 10 using electroconvulsiometer. Change in duration of hind limb extension was measured. In the pentylenetetrazole (PTZ) method, PTZ at a dose of 60 mg/kg i.p was given to produce convulsion in all mice. A total of 30 mice were divided into five groups of six animals. The control group received orally 2 ml of distilled water daily for 15 days. The standard group received diazepam 4 mg/kg i.p. daily for 15 days. The test group received orally NLE at the dose of 200, 400, and 800 mg/kg for 15 days. The onset of clonic convulsions and the total number of clonic convulsions in 20 min was observed.

Results: The mean duration of hind limb extension in test group before the highest dose of neem extract (800 mg) administration was 8.7 ± 0.3 and after 15 days it was 8.8 ± 0.21. The mean onset of convulsions and mean number of convulsions in 20 min in the test group with highest dose of neem extract (800 mg) administration was 3.44 ± 0.24 and 42 ± 0.76, respectively, and there was no significant difference when compared to the control group.

Conclusion: The alcoholic extract of Azadirachta indica (neem) root is not having any anticonvulsant activity in rodents.

Key words: Neem; Rats; Mice; Experimental Pharmacology; Epilepsy; Plant Extract


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Rohit Dixit
Articles by Prabhakar Raosaheb Patil
on Google
on Google Scholar
Article Statistics
 Viewed: 459
Downloaded: 251
Cited: 0

How to Cite this Article
Pubmed Style

Dixit R, Patil PR. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol. 2018; 8(8): 1171-1174. doi:10.5455/njppp.2018.8.0310626042018


Web Style

Dixit R, Patil PR. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. http://www.njppp.com/?mno=295094 [Access: September 19, 2018]. doi:10.5455/njppp.2018.8.0310626042018


AMA (American Medical Association) Style

Dixit R, Patil PR. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol. 2018; 8(8): 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Vancouver/ICMJE Style

Dixit R, Patil PR. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol. (2018), [cited September 19, 2018]; 8(8): 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Harvard Style

Dixit, R. & Patil, P. R. (2018) Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. Natl J Physiol Pharm Pharmacol, 8 (8), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Turabian Style

Dixit, Rohit, and Prabhakar Raosaheb Patil. 2018. Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. National Journal of Physiology, Pharmacy and Pharmacology, 8 (8), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



Chicago Style

Dixit, Rohit, and Prabhakar Raosaheb Patil. "Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents." National Journal of Physiology, Pharmacy and Pharmacology 8 (2018), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018



MLA (The Modern Language Association) Style

Dixit, Rohit, and Prabhakar Raosaheb Patil. "Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents." National Journal of Physiology, Pharmacy and Pharmacology 8.8 (2018), 1171-1174. Print. doi:10.5455/njppp.2018.8.0310626042018



APA (American Psychological Association) Style

Dixit, R. & Patil, P. R. (2018) Alcoholic extract of Azadirachta indica (Neem) root has no anticonvulsant activity in rodents. National Journal of Physiology, Pharmacy and Pharmacology, 8 (8), 1171-1174. doi:10.5455/njppp.2018.8.0310626042018





Most Viewed Articles
  • Applications of computer science in Pharmacy: An overview
    Shivraj Popat Jadhav, Komal Nikam, Anand Gandhi, Kishor Salunkhe, Narendra Shinde.
    Natl J Physiol Pharm Pharmacol. 2012; 2(1): 1-9
    » Abstract

  • How to calculate sample size in animal studies?
    Hitesh Shah
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 35-39
    » Abstract

  • Chlorhexidine:The gold standard in chemical plaque control [English]
    Setu mathur, Tanu mathur,Rahul shrivastava, Rohit khatri
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 45-50
    » Abstract

  • Assessment of Nutritional Status of Pregnant Women Attending the City Tebessa PMI (Algeria) [English]
    Taleb Salima, Kaibi Mounira, Deghboudj Nadjia
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 97-105
    » Abstract

  • Analyzing Pharmaceutical Industry [English]
    Veselin Tima Dickov, Dragan Mitrovic, Boris Milan Kuzman
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 1-8
    » Abstract & References » doi: 10.5455/njppp.2011.1.1-8

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138

  • Second MBBS medical student’s feedback on teaching methodology and evaluation methods in Pharmacology
    Nilesh Chavda, Preeti Yadav, Jay Karan, N. D. Kantharia
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 23-31
    » Abstract & References » doi: 10.5455/njppp.2011.1.23-31

  • Prophylaxis and Treatment for Leptospirosis: Where are the Evidences?
    Jaykaran Charan, Deepak Saxena, Summaiya Mulla
    Natl J Physiol Pharm Pharmacol. 2012; 2(2): 78-83
    » Abstract & References » doi: 10.5455/njppp.2012.2.78-83

  • How to write a thesis? [English]
    Swapnil s Agarwal, Preeti Yadav, Krishnadutt Chavali, Lavlesh Kumar
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 86-90
    » Abstract

  • Most Downloaded
  • How to calculate sample size in animal studies?
    Hitesh Shah
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 35-39
    » Abstract

  • Chlorhexidine:The gold standard in chemical plaque control [English]
    Setu mathur, Tanu mathur,Rahul shrivastava, Rohit khatri
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 45-50
    » Abstract

  • Applications of computer science in Pharmacy: An overview
    Shivraj Popat Jadhav, Komal Nikam, Anand Gandhi, Kishor Salunkhe, Narendra Shinde.
    Natl J Physiol Pharm Pharmacol. 2012; 2(1): 1-9
    » Abstract

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114

  • Analyzing Pharmaceutical Industry [English]
    Veselin Tima Dickov, Dragan Mitrovic, Boris Milan Kuzman
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 1-8
    » Abstract & References » doi: 10.5455/njppp.2011.1.1-8

  • Prophylaxis and Treatment for Leptospirosis: Where are the Evidences?
    Jaykaran Charan, Deepak Saxena, Summaiya Mulla
    Natl J Physiol Pharm Pharmacol. 2012; 2(2): 78-83
    » Abstract & References » doi: 10.5455/njppp.2012.2.78-83

  • Second MBBS medical student’s feedback on teaching methodology and evaluation methods in Pharmacology
    Nilesh Chavda, Preeti Yadav, Jay Karan, N. D. Kantharia
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 23-31
    » Abstract & References » doi: 10.5455/njppp.2011.1.23-31

  • A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.
    Chetankumar R. Acharya, Hetal N. Thakar, S. K. Vajpeyee
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 14-20
    » Abstract & References » doi: 10.5455/njppp.2013.3.14-20

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138

  • Assessment of Nutritional Status of Pregnant Women Attending the City Tebessa PMI (Algeria) [English]
    Taleb Salima, Kaibi Mounira, Deghboudj Nadjia
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 97-105
    » Abstract

  • Most Cited Articles
  • A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.
    Chetankumar R. Acharya, Hetal N. Thakar, S. K. Vajpeyee
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 14-20
    » Abstract & References » doi: 10.5455/njppp.2013.3.14-20
    Cited : 8 times [Click to see citing articles]

  • A pilot study to evaluate the effect of HAART on gonadal dysfunction in male HIV patients
    Abhishek Pathak, Lalit Prashant Meena, Jaya Chakravarty, Madhukar Rai, Shyam Sundar
    Natl J Physiol Pharm Pharmacol. 2015; 5(1): 33-35
    » Abstract & References » doi: 10.5455/njppp.2015.5.120720142
    Cited : 6 times [Click to see citing articles]

  • Quercetin improved spatial memory dysfunctions in rat model of intracerebroventricular streptozotocin-induced sporadic Alzheimer’s disease
    Manouchehr Ashrafpour, Sahar Parsaei, Hamid Sepehri
    Natl J Physiol Pharm Pharmacol. 2015; 5(5): 411-415
    » Abstract & References » doi: 10.5455/njppp.2015.5.2308201563
    Cited : 6 times [Click to see citing articles]

  • Newer anti-platelet and anti-thrombotic drugs on the horizon
    Ajai Garg, Anjana Pandey, Pranab Oza, Mridul Chaturvedi
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 105-110
    » Abstract & References » doi: 10.5455/njppp.2013.3.090420131
    Cited : 5 times [Click to see citing articles]

  • Rate pressure product predicts cardiovascular risk in type 2 diabetics with cardiac autonomic neuropathy
    Rishu Segan, Vidushi Gupta, Lily Walia, Naveen Mittal
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 43-47
    » Abstract & References » doi: 10.5455/njppp.2013.3.43-47
    Cited : 4 times [Click to see citing articles]

  • Effects of sixteen weeks exercise training on left ventricular dimensions and function in young athletes
    Sandip M Hulke, Yuganti P Vaidya, Amandeepkour R Ratta
    Natl J Physiol Pharm Pharmacol. 2012; 2(2): 152-158
    » Abstract & References » doi: 10.5455/njppp.2012.2.152-158
    Cited : 4 times [Click to see citing articles]

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138
    Cited : 4 times [Click to see citing articles]

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114
    Cited : 4 times [Click to see citing articles]

  • Prevalence and risk factors for eating disorders in Indian adolescent females
    Amit A Upadhyah, Rajesh Misra, Deepak N Parchwani, Pankaj B Maheria
    Natl J Physiol Pharm Pharmacol. 2014; 4(2): 153-157
    » Abstract & References » doi: 10.5455/njppp.2014.4.041220131
    Cited : 4 times [Click to see citing articles]

  • Time domain measures of heart rate variability during acute mental stress in Type 2 diabetics- A case control study
    Anupama Deepak, Deepak A N, Shobha Nallulwar, Vitthal Khode
    Natl J Physiol Pharm Pharmacol. 2014; 4(1): 34-38
    » Abstract & References » doi: 10.5455/njppp.2014.4.080720131
    Cited : 4 times [Click to see citing articles]