E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research

Online Publishing Date:
22 / 11 / 2022

 


A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry

Srishtidhar Mangal, Samar Kumar Barai, Sabyasachi Choudhury, Ipsita Saha.


Abstract
Background: Body cavity fluids are evaluated by cell block (CB) technique. In conventional cytological smears (CS), appropriate identification of the cells as either reactive mesothelial cells or malignant cells is a diagnostic problem. In CB method, there is increase in cellularity, so it gives more morphological details of cells and improving the sensitivity of diagnosis. Sections obtained by CB method are also can be stored for future study like immunohistochemistry (IHC).

Aims and Objectives: The aim of the study was to determine the diagnostic efficacy of CB technique over conventional CS and to study of IHC in CB of malignant pleural effusion.

Materials and Methods: This will be institution-based, cross-sectional, and observational study. The pleural fluid (except blood and pus) obtained by thoracocentesis from both male and female patient (>18 years of age) 100 cases was included in the study. Pleural fluid mixed with blood/pus, fluid from cystic lesion, and inadequate sample (<15 ml vol.) were excluded in the study.

Results: Association between architectural pattern in CB versus architectural pattern in cytology smear showed statistically significant value Chi-square: 201.7417, P < 0.0001.

Conclusion: We may conclude that CB technique is better than conventional cytology smear technique especially in case of malignant pleural effusion. It increases true positive cases and decreases false negative cases. It also increases cellularity, maintain architectural pattern, and cellular morphology.

Key words: Pleural Effusion; Cell Block; Conventional Cytology Smear


 
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How to Cite this Article
Pubmed Style

Mangal S, Barai SK, Choudhury S, Saha I. A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry. Natl J Physiol Pharm Pharmacol. 2023; 13(6): 1183-1187. doi:10.5455/njppp.2023.13.08414202214112022


Web Style

Mangal S, Barai SK, Choudhury S, Saha I. A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry. https://www.njppp.com/?mno=109941 [Access: March 14, 2024]. doi:10.5455/njppp.2023.13.08414202214112022


AMA (American Medical Association) Style

Mangal S, Barai SK, Choudhury S, Saha I. A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry. Natl J Physiol Pharm Pharmacol. 2023; 13(6): 1183-1187. doi:10.5455/njppp.2023.13.08414202214112022



Vancouver/ICMJE Style

Mangal S, Barai SK, Choudhury S, Saha I. A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry. Natl J Physiol Pharm Pharmacol. (2023), [cited March 14, 2024]; 13(6): 1183-1187. doi:10.5455/njppp.2023.13.08414202214112022



Harvard Style

Mangal, S., Barai, . S. K., Choudhury, . S. & Saha, . I. (2023) A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry. Natl J Physiol Pharm Pharmacol, 13 (6), 1183-1187. doi:10.5455/njppp.2023.13.08414202214112022



Turabian Style

Mangal, Srishtidhar, Samar Kumar Barai, Sabyasachi Choudhury, and Ipsita Saha. 2023. A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry. National Journal of Physiology, Pharmacy and Pharmacology, 13 (6), 1183-1187. doi:10.5455/njppp.2023.13.08414202214112022



Chicago Style

Mangal, Srishtidhar, Samar Kumar Barai, Sabyasachi Choudhury, and Ipsita Saha. "A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry." National Journal of Physiology, Pharmacy and Pharmacology 13 (2023), 1183-1187. doi:10.5455/njppp.2023.13.08414202214112022



MLA (The Modern Language Association) Style

Mangal, Srishtidhar, Samar Kumar Barai, Sabyasachi Choudhury, and Ipsita Saha. "A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry." National Journal of Physiology, Pharmacy and Pharmacology 13.6 (2023), 1183-1187. Print. doi:10.5455/njppp.2023.13.08414202214112022



APA (American Psychological Association) Style

Mangal, S., Barai, . S. K., Choudhury, . S. & Saha, . I. (2023) A comparative study of conventional cytology and cell block in the diagnosis of pleural effusion with special reference to immunohistochemistry. National Journal of Physiology, Pharmacy and Pharmacology, 13 (6), 1183-1187. doi:10.5455/njppp.2023.13.08414202214112022