E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri.

Abstract
Background: Poor wound healing is a complication of diabetes mellitus. Various agents have been used to promote wound healing in diabetic patients. Papain urea is used as a sloughing agent, whereas sodium fusidate and calcium mupirocin are used as topical antimicrobials for wounds in diabetics.

Aims and Objective: To evaluate effect of topical sodium fusidate, calcium mupirocin, and papain–urea on wound healing in diabetic wistar rats.

Materials and Methods: Incision and excision wound models were used. Each model had five groups of six rats each and one group was nondiabetic control. Diabetes was induced in the remaining four groups, one diabetic control, and three test groups in each model, using streptozotocin (30 mg/kg intraperitoneally). Wounds were made in diabetic rats with blood glucose levels of more than 250 mg/dL. Paraffin ointment base was applied to wounds of nondiabetic and diabetic control, whereas sodium fusidate, mupirocin, and papain–urea were applied topically to three test groups, respectively, in each model.

Result: In sodium fusidate-treated group, there was a significant increase in breaking strength of incision wound, contraction rate, and hydroxyproline content of excision wound as compared to diabetic control group. There was significant decrease in period of epithelization in sodium fusidate-treated group compared to diabetic control group. There was no significant alteration in wound healing by mupirocin and papain–urea.

Conclusion: Topical application of sodium fusidate promoted wound healing in diabetic rats.

Key words: Breaking strength; Epithelization; Wound contraction; Streptozotocin


 
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REFERENCES
1. Bitar MS. Insulin-like growth factor-1 reverses diabetes-induced wound healing impairment in rats. Horm Metab Res. 1997;29(8): 383-6. [DOI via Crossref]    [Pubmed]   
2. Luzi L. Pancreas transplantation and diabetic complications. N Engl J Med. 1998;339(2):115-7. [DOI via Crossref]    [Pubmed]   
3. Galkowska H, Wojewodzka U, Olszewski WL. Chemokines, cytokines, and growth factors in keratinocytes and dermal endothelial cells in the margin of chronic diabetic foot ulcers. Wound Repair Regen. 2006;14:558-65. [DOI via Crossref]    [Pubmed]   
4. Galiano RD, Tepper OM, Pelo CR, Bhatt KA, Callaghan M, Bastidas N, et al. Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells. Am J Pathol. 2004;164(6):1935-47. [DOI via Crossref]   
5. Maruyama K, Asai J, Ii M, Thorne T, Losordo DW, D’Amore PA. Decreased macrophage number and activation lead to reduced lymphatic vessel formation and contribute to impaired diabetic would healing. Am J Pathol. 2007;170(4): 1178-91. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
6. Alvarez OM, Fernandez-Obregon A, Rogers RS, Bergamo L, Masso J, Black M. Chemical debridement of pressure ulcers: a prospective, randomized, comparative trial of collagenase and papain-urea formulations. Wounds. 2000;12(2):15-25.
7. Rathur H, Rajbhandari SM. Prevention of diabetic foot ulcers: holy grail of foot clinics. Int J Diabetes Metab. 2003;11:56-61.
8. Vijaykumar H, Pai SA, Pandey V, Kamble PK. Comparative study of collagenase and papain-urea based preparations in the management of chronic non healing limb ulcers. Indian J Sci Technol. 2011;4(9):1096-1100.
9. Lipsky BA, Hoey C. Topical antimicrobial therapy for treating chronic wounds. Clin Infect Dis. 2009;49(10):1541-9. [DOI via Crossref]    [Pubmed]   
10. Spelman D. Fusidic acid in skin and soft tissue infections Int J Antimicrob Agents. 1999;12(Suppl 2):S59-66. [DOI via Crossref]   
11. Wilkinson JD. Fusidic acid in dermatology Br J Dermatol. 1998; 139(Suppl 53):S37-40. [DOI via Crossref]   
12. Fuller AT, Mellows G, Woolford M, Banks GT, Barrow KD, Chain EB. Pseudomonic acid: an antibiotic produced by Pseudomonas fluorescens. Nature. 1971;234(5329):416-7. [DOI via Crossref]    [Pubmed]   
13. Bairy KL, Nayak V, Adiga S. Comparative effect of Sodium fusidate, Framycetin and Calcium mupirocin on experimentally induced burn wound healing. Int J Pharm Sci Bio. 2010;1(2):100-2.
14. Williford PM. Opportunities for mupirocin calcium cream in the emergency department. J Emerg Med. 1999;17(1):213-20. [DOI via Crossref]   
15. Sundaram EN, Reddy PU, Singh KP. Effect of alcoholic extracts of Indian medicinal plants on the altered enzymatic activities of diabetic rats. Indian J Pharm Sci. 2009;71(5):594-8. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
16. Nayak SB, Pinto PL, Maharaj D. Wound healing activity of Carica papaya L. in experimentally induced diabetic rats. Indian J Exp Biol. 2007;45(8):739-43. [Pubmed]   
17. Ehrlich HP, Hunt TK. Effects of cortisone and vitamin A on wound healing. Ann Surg. 1968;167(3):324-8. [DOI via Crossref]   
18. Lee KH. Studies on the mechanism of action of salicylates.3. Effect of vitamin A on the wound healing retardation action of aspirin. J Pharm Sci. 1968;57(7):1238-40. [DOI via Crossref]    [Pubmed]   
19. Morton JJ, Malone MH. Evaluation of vulneray activity by an open wound procedure in rats. Arch Int Pharmacodyn Ther. 1972; 196(1):117-26. [Pubmed]   
20. Kumar MS, Kirubanandan S, Sripriya R, Sehgal PK. Triphala promotes healing of infected full-thickness dermal wound. J Surg Res. 2008;144(1):94-101. [DOI via Crossref]    [Pubmed]   
21. Gutierrez RM, Mitchell S, Solis RV. Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2008;117(1):1-27. [DOI via Crossref]    [Pubmed]   
22. Neuman RE, Logan MA. The determination of collagen and elastin in tissues. J Biol Chem. 1950;186(2):549-56. [Pubmed]   
23. Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-22. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
24. Rasik AM, Shukla A. Antioxidant status in delayed healing type of wounds. Int J Exp Pathol. 2000;81(4):257-63. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
25. Bitar MS, Labbad ZN. Transforming growth factor-beta and insulinlike growth factor-I in relation to diabetes-induced impairment of wound healing. J Surg Res. 1996;61(1):113-9. [DOI via Crossref]    [Pubmed]   
26. Beer HD, Longaker MT, Werner S. Reduced expression of PDGF and PDGF receptors during impaired wound healing. J Invest Dermatol. 1997;109(2):132-8. [DOI via Crossref]    [Pubmed]   
27. Hehenberger K, Heilborn JD, Brismar K, Hansson A. Inhibited proliferation of fibroblasts derived from chronic diabetic wounds and normal dermal fibroblasts treated with high glucose is associated with increased formation of l-lactate. Wound Repair Regen. 1998;6(2):135-41. [DOI via Crossref]    [Pubmed]   
28. Darby IA, Bisucci T, Hewitson TD, MacLellan DG. Apoptosis is increased in a model of diabetes -impaired wound healing in genetically diabetic mice. Int J Biochem Cell Biol. 1997;29(1):191-200. [DOI via Crossref]   
29. Spanheimer RG, Umpierrez GE, Stumpf V. Decreased collagen production in diabetic rats. Diabetes. 1988;37(4):371-6. [DOI via Crossref]    [Pubmed]   
30. Perez GRM, Vargas SR, Ortiz HYD. Wound healing properties of Hylocereus undatus on diabetic rats. Phytother Res. 2005;19(8):665-8. [DOI via Crossref]    [Pubmed]   
31. McAnulty RJ. Methods for measuring hydroxyproline and estimating in vivo rates of collagen synthesis and degradation. Methods Mol Med. 2005;117:189-207. [Pubmed]   
32. Mast BA. The skin In:(Eds.) Wound Healing: Biochemical and Clinical Aspects. 1st edn Philadelphia, PA: Saunders, 1992. pp. 344-55.
33. Ilango K, Chitra V. Wound healing and antioxidant activities of the fruit pulp of Limonia acidissima Linn (Rutaceae) in rats. Trop J Pharm Res. 2010;9(3):223-30. [DOI via Crossref]   
34. Lee JH, Lim SJ, Oh DH, Ku SK, Li DX, Yong CS, et al. Wound healing evaluation of sodium fucidate-loaded polyvinylalcohol /sodium carboxymethylcellulose-based wound dressing. Arch Pharm Res. 2010;33(7):1083-9. [DOI via Crossref]    [Pubmed]   

How to Cite this Article
Pubmed Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri. Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats. Natl J Physiol Pharm Pharmacol. 2016; 6(3): 209-214. doi:10.5455/njppp.2016.6.28012016115


Web Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri. Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats. https://www.njppp.com/?mno=216631 [Access: June 01, 2022]. doi:10.5455/njppp.2016.6.28012016115


AMA (American Medical Association) Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri. Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats. Natl J Physiol Pharm Pharmacol. 2016; 6(3): 209-214. doi:10.5455/njppp.2016.6.28012016115



Vancouver/ICMJE Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri. Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats. Natl J Physiol Pharm Pharmacol. (2016), [cited June 01, 2022]; 6(3): 209-214. doi:10.5455/njppp.2016.6.28012016115



Harvard Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri (2016) Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats. Natl J Physiol Pharm Pharmacol, 6 (3), 209-214. doi:10.5455/njppp.2016.6.28012016115



Turabian Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri. 2016. Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (3), 209-214. doi:10.5455/njppp.2016.6.28012016115



Chicago Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri. "Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 6 (2016), 209-214. doi:10.5455/njppp.2016.6.28012016115



MLA (The Modern Language Association) Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri. "Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 6.3 (2016), 209-214. Print. doi:10.5455/njppp.2016.6.28012016115



APA (American Psychological Association) Style

Smita Shenoy, Raghu Murthy, Lalit Mohan, Amoolya Gowda, Venu Madhav Nelluri (2016) Effect of topical sodium fusidate, calcium mupirocin and papain—urea on wound healing in diabetic wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (3), 209-214. doi:10.5455/njppp.2016.6.28012016115