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Original Research 


Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity

Volha Schädler, Schanna Dergatschewa.

Abstract
Background: Rubus caesius L. is used in traditional medicine, but pharmacological activity data and standardization methods are lacking.

Aims and Objectives: This study sought to conduct a pharmacognostic analysis of R. caesius L. leaves and assess these leaves’ hypoglycemic activity.

Materials and Methods: External, anatomical, and diagnostic features of the leaves were studied in accordance with the State Pharmacopoeia of the Republic of Belarus T1. Conventional qualitative thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used. The hypoglycemic activity of the aqueous extract of the leaves was investigated in an alloxan-induced diabetes model.

Results: External, anatomical, and diagnostic features of the leaves were determined. The stomata were anomocytic; calcium oxalate crystals were observed in the mesophyll; stellate-arrayed hairs, star-arrayed hairs, capitate hairs, and simple unicellular hairs with a helical fold were identified. Qualitative results indicated that biologically active substances, such as flavonoids and tannins, were present in R. caesius L. leaves. HPLC and TLC revealed the presence of hyperoside in these leaves. Soundness indicators were defined: The weight loss on drying the R. caesius L. leaves varied from 8.61% to 9.52%, and the average weight loss was 9.1%; determinations of total ash varied from 5.40% to 6.50%; determinations of ash insoluble in hydrochloric acid varied from 0.12% to 0.13%. The hypoglycemic activity of an aqueous extract of R. caesius L. leaves in a 500 mg/kg draught was determined in a rat model of alloxan-induced diabetes.

Conclusion: The data obtained here can be used to develop regulatory documentation for a new type of medicinal plant tissue: R. caesius L. leaves.

Key words: Pharmacognostic; Phytochemical; Hyperoside; Standardization; Hypoglycemic Activity


 
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Pubmed Style

Volha Schädler, Schanna Dergatschewa. Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity. Natl J Physiol Pharm Pharmacol. 2017; 7(5): 501-508. doi:10.5455/njppp.2017.7.1234224012017


Web Style

Volha Schädler, Schanna Dergatschewa. Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity. http://www.njppp.com/?mno=252623 [Access: January 01, 2022]. doi:10.5455/njppp.2017.7.1234224012017


AMA (American Medical Association) Style

Volha Schädler, Schanna Dergatschewa. Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity. Natl J Physiol Pharm Pharmacol. 2017; 7(5): 501-508. doi:10.5455/njppp.2017.7.1234224012017



Vancouver/ICMJE Style

Volha Schädler, Schanna Dergatschewa. Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity. Natl J Physiol Pharm Pharmacol. (2017), [cited January 01, 2022]; 7(5): 501-508. doi:10.5455/njppp.2017.7.1234224012017



Harvard Style

Volha Schädler, Schanna Dergatschewa (2017) Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity. Natl J Physiol Pharm Pharmacol, 7 (5), 501-508. doi:10.5455/njppp.2017.7.1234224012017



Turabian Style

Volha Schädler, Schanna Dergatschewa. 2017. Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity. National Journal of Physiology, Pharmacy and Pharmacology, 7 (5), 501-508. doi:10.5455/njppp.2017.7.1234224012017



Chicago Style

Volha Schädler, Schanna Dergatschewa. "Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity." National Journal of Physiology, Pharmacy and Pharmacology 7 (2017), 501-508. doi:10.5455/njppp.2017.7.1234224012017



MLA (The Modern Language Association) Style

Volha Schädler, Schanna Dergatschewa. "Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity." National Journal of Physiology, Pharmacy and Pharmacology 7.5 (2017), 501-508. Print. doi:10.5455/njppp.2017.7.1234224012017



APA (American Psychological Association) Style

Volha Schädler, Schanna Dergatschewa (2017) Rubus caesius L. leaves: Pharmacognostic analysis and the study of hypoglycemic activity. National Journal of Physiology, Pharmacy and Pharmacology, 7 (5), 501-508. doi:10.5455/njppp.2017.7.1234224012017