E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?

Raudhahtulnur Mohd Zainudin, Fazlin Zaini, Resni Mona, Jannathul Firdous, Noorzaid Muhamad.

Abstract
Background: GLUT4 acts as the insulin-responsive glucose transporter, thus promoting the uptake of glucose from circulation into muscle and adipose tissue.

Aims and Objectives: The present study was conducted to determine the effect Orthosiphon stamineus Benth. extract on GLUT4 translocation in the skeletal muscle of type II diabetic rats using immunofluorescent technique.

Materials and Methods: Male Wistar rats aged 10 weeks (180–220 g) were divided into four groups: Normal control group (vehicle receiving group), positive control group (diabetic rats treated with 5 mg/kg metformin), negative control group (diabetic rats not receiving any treatment), and treated group (diabetic rats treated with 1 g/kg O. stamineus Benth. aqueous leaf extract). The rats were treated daily for 14 days where the fasting blood glucose level was measured daily; meanwhile, serum insulin was measured before (after injected with STZ-NAD) and after they get treated (at the end of 14 days). At the end of the treatment, the rats were sacrificed and soleus muscles were dissected, fixed in 10% buffered formalin, and processed for immunofluorescence technique.

Results: The findings showed significantly reduced (P < 0.01) mean fasting glucose concentration in positive control and treated groups. Relative pancreas weight and serum plasma concentration were similar in all groups. In the diabetic rats treated with O. stamineus Benth., improvement in the translocation activity of GLUT4 was observed.

Conclusion: Our findings suggest that O. stamineus Benth. has the potential in the treatment of Type II diabetes mellitus though the improvement of GLUT4 translocation activity.

Key words: Diabetes Mellitus; GLUT 4; Immunofluorescent Technique; Metformin; Orthosiphon stamineus Benth


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Raudhahtulnur Mohd Zainudin
Articles by Fazlin Zaini
Articles by Resni Mona
Articles by Jannathul Firdous
Articles by Noorzaid Muhamad
on Google
on Google Scholar
Article Statistics
 Viewed: 484
Downloaded: 237
Cited: 0

How to Cite this Article
Pubmed Style

Zainudin RM, Zaini F, Mona R, Firdous J, Muhamad N. Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?. Natl J Physiol Pharm Pharmacol. 2018; 8(7): 1061-1064. doi:10.5455/njppp.2018.8.1041326032018


Web Style

Zainudin RM, Zaini F, Mona R, Firdous J, Muhamad N. Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?. http://www.njppp.com/?mno=281458 [Access: September 19, 2018]. doi:10.5455/njppp.2018.8.1041326032018


AMA (American Medical Association) Style

Zainudin RM, Zaini F, Mona R, Firdous J, Muhamad N. Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?. Natl J Physiol Pharm Pharmacol. 2018; 8(7): 1061-1064. doi:10.5455/njppp.2018.8.1041326032018



Vancouver/ICMJE Style

Zainudin RM, Zaini F, Mona R, Firdous J, Muhamad N. Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?. Natl J Physiol Pharm Pharmacol. (2018), [cited September 19, 2018]; 8(7): 1061-1064. doi:10.5455/njppp.2018.8.1041326032018



Harvard Style

Zainudin, R. M., Zaini, F., Mona, R., Firdous, J. & Muhamad, N. (2018) Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?. Natl J Physiol Pharm Pharmacol, 8 (7), 1061-1064. doi:10.5455/njppp.2018.8.1041326032018



Turabian Style

Zainudin, Raudhahtulnur Mohd, Fazlin Zaini, Resni Mona, Jannathul Firdous, and Noorzaid Muhamad. 2018. Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?. National Journal of Physiology, Pharmacy and Pharmacology, 8 (7), 1061-1064. doi:10.5455/njppp.2018.8.1041326032018



Chicago Style

Zainudin, Raudhahtulnur Mohd, Fazlin Zaini, Resni Mona, Jannathul Firdous, and Noorzaid Muhamad. "Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?." National Journal of Physiology, Pharmacy and Pharmacology 8 (2018), 1061-1064. doi:10.5455/njppp.2018.8.1041326032018



MLA (The Modern Language Association) Style

Zainudin, Raudhahtulnur Mohd, Fazlin Zaini, Resni Mona, Jannathul Firdous, and Noorzaid Muhamad. "Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?." National Journal of Physiology, Pharmacy and Pharmacology 8.7 (2018), 1061-1064. Print. doi:10.5455/njppp.2018.8.1041326032018



APA (American Psychological Association) Style

Zainudin, R. M., Zaini, F., Mona, R., Firdous, J. & Muhamad, N. (2018) Does Orthosiphon stamineus Benth. enhance GLUT4 translocation in the skeletal muscle of induced type II diabetic rats?. National Journal of Physiology, Pharmacy and Pharmacology, 8 (7), 1061-1064. doi:10.5455/njppp.2018.8.1041326032018





Most Viewed Articles
  • Applications of computer science in Pharmacy: An overview
    Shivraj Popat Jadhav, Komal Nikam, Anand Gandhi, Kishor Salunkhe, Narendra Shinde.
    Natl J Physiol Pharm Pharmacol. 2012; 2(1): 1-9
    » Abstract

  • How to calculate sample size in animal studies?
    Hitesh Shah
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 35-39
    » Abstract

  • Chlorhexidine:The gold standard in chemical plaque control [English]
    Setu mathur, Tanu mathur,Rahul shrivastava, Rohit khatri
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 45-50
    » Abstract

  • Assessment of Nutritional Status of Pregnant Women Attending the City Tebessa PMI (Algeria) [English]
    Taleb Salima, Kaibi Mounira, Deghboudj Nadjia
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 97-105
    » Abstract

  • Analyzing Pharmaceutical Industry [English]
    Veselin Tima Dickov, Dragan Mitrovic, Boris Milan Kuzman
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 1-8
    » Abstract & References » doi: 10.5455/njppp.2011.1.1-8

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138

  • Second MBBS medical student’s feedback on teaching methodology and evaluation methods in Pharmacology
    Nilesh Chavda, Preeti Yadav, Jay Karan, N. D. Kantharia
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 23-31
    » Abstract & References » doi: 10.5455/njppp.2011.1.23-31

  • Prophylaxis and Treatment for Leptospirosis: Where are the Evidences?
    Jaykaran Charan, Deepak Saxena, Summaiya Mulla
    Natl J Physiol Pharm Pharmacol. 2012; 2(2): 78-83
    » Abstract & References » doi: 10.5455/njppp.2012.2.78-83

  • How to write a thesis? [English]
    Swapnil s Agarwal, Preeti Yadav, Krishnadutt Chavali, Lavlesh Kumar
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 86-90
    » Abstract

  • Most Downloaded
  • How to calculate sample size in animal studies?
    Hitesh Shah
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 35-39
    » Abstract

  • Chlorhexidine:The gold standard in chemical plaque control [English]
    Setu mathur, Tanu mathur,Rahul shrivastava, Rohit khatri
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 45-50
    » Abstract

  • Applications of computer science in Pharmacy: An overview
    Shivraj Popat Jadhav, Komal Nikam, Anand Gandhi, Kishor Salunkhe, Narendra Shinde.
    Natl J Physiol Pharm Pharmacol. 2012; 2(1): 1-9
    » Abstract

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114

  • Analyzing Pharmaceutical Industry [English]
    Veselin Tima Dickov, Dragan Mitrovic, Boris Milan Kuzman
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 1-8
    » Abstract & References » doi: 10.5455/njppp.2011.1.1-8

  • Prophylaxis and Treatment for Leptospirosis: Where are the Evidences?
    Jaykaran Charan, Deepak Saxena, Summaiya Mulla
    Natl J Physiol Pharm Pharmacol. 2012; 2(2): 78-83
    » Abstract & References » doi: 10.5455/njppp.2012.2.78-83

  • Second MBBS medical student’s feedback on teaching methodology and evaluation methods in Pharmacology
    Nilesh Chavda, Preeti Yadav, Jay Karan, N. D. Kantharia
    Natl J Physiol Pharm Pharmacol. 2011; 1(1): 23-31
    » Abstract & References » doi: 10.5455/njppp.2011.1.23-31

  • A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.
    Chetankumar R. Acharya, Hetal N. Thakar, S. K. Vajpeyee
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 14-20
    » Abstract & References » doi: 10.5455/njppp.2013.3.14-20

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138

  • Assessment of Nutritional Status of Pregnant Women Attending the City Tebessa PMI (Algeria) [English]
    Taleb Salima, Kaibi Mounira, Deghboudj Nadjia
    Natl J Physiol Pharm Pharmacol. 2011; 1(2): 97-105
    » Abstract

  • Most Cited Articles
  • A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant vitamin C in Wistar rats.
    Chetankumar R. Acharya, Hetal N. Thakar, S. K. Vajpeyee
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 14-20
    » Abstract & References » doi: 10.5455/njppp.2013.3.14-20
    Cited : 8 times [Click to see citing articles]

  • A pilot study to evaluate the effect of HAART on gonadal dysfunction in male HIV patients
    Abhishek Pathak, Lalit Prashant Meena, Jaya Chakravarty, Madhukar Rai, Shyam Sundar
    Natl J Physiol Pharm Pharmacol. 2015; 5(1): 33-35
    » Abstract & References » doi: 10.5455/njppp.2015.5.120720142
    Cited : 6 times [Click to see citing articles]

  • Quercetin improved spatial memory dysfunctions in rat model of intracerebroventricular streptozotocin-induced sporadic Alzheimer’s disease
    Manouchehr Ashrafpour, Sahar Parsaei, Hamid Sepehri
    Natl J Physiol Pharm Pharmacol. 2015; 5(5): 411-415
    » Abstract & References » doi: 10.5455/njppp.2015.5.2308201563
    Cited : 6 times [Click to see citing articles]

  • Newer anti-platelet and anti-thrombotic drugs on the horizon
    Ajai Garg, Anjana Pandey, Pranab Oza, Mridul Chaturvedi
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 105-110
    » Abstract & References » doi: 10.5455/njppp.2013.3.090420131
    Cited : 5 times [Click to see citing articles]

  • Rate pressure product predicts cardiovascular risk in type 2 diabetics with cardiac autonomic neuropathy
    Rishu Segan, Vidushi Gupta, Lily Walia, Naveen Mittal
    Natl J Physiol Pharm Pharmacol. 2013; 3(1): 43-47
    » Abstract & References » doi: 10.5455/njppp.2013.3.43-47
    Cited : 4 times [Click to see citing articles]

  • Effects of sixteen weeks exercise training on left ventricular dimensions and function in young athletes
    Sandip M Hulke, Yuganti P Vaidya, Amandeepkour R Ratta
    Natl J Physiol Pharm Pharmacol. 2012; 2(2): 152-158
    » Abstract & References » doi: 10.5455/njppp.2012.2.152-158
    Cited : 4 times [Click to see citing articles]

  • Antidiabetic activity of alcoholic extract of Neem (Azadirachta Indica) root bark
    Prabhakar Patil, Sudha Patil, Abhay Mane, Sushilkumar Verma
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 142-146
    » Abstract & References » doi: 10.5455/njppp.2013.3.134-138
    Cited : 4 times [Click to see citing articles]

  • Difference in pulmonary function test among the athletic and sedentary population
    ShobhaRani Vedala, Niranjan Paul, Abhay B Mane
    Natl J Physiol Pharm Pharmacol. 2013; 3(2): 118-123
    » Abstract & References » doi: 10.5455/njppp.2013.3.109-114
    Cited : 4 times [Click to see citing articles]

  • Prevalence and risk factors for eating disorders in Indian adolescent females
    Amit A Upadhyah, Rajesh Misra, Deepak N Parchwani, Pankaj B Maheria
    Natl J Physiol Pharm Pharmacol. 2014; 4(2): 153-157
    » Abstract & References » doi: 10.5455/njppp.2014.4.041220131
    Cited : 4 times [Click to see citing articles]

  • Time domain measures of heart rate variability during acute mental stress in Type 2 diabetics- A case control study
    Anupama Deepak, Deepak A N, Shobha Nallulwar, Vitthal Khode
    Natl J Physiol Pharm Pharmacol. 2014; 4(1): 34-38
    » Abstract & References » doi: 10.5455/njppp.2014.4.080720131
    Cited : 4 times [Click to see citing articles]