E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya.

Cited by (1)

Abstract
Background: Cadmium (Cd), an environmental metallic toxicant with varying degrees of toxicity, exists in different oxidational or transitional states and causes various blood disorders. Cadmium (Cd) is known to affect various organs on both acute and chronic exposures. Studies have revealed that the most important tissue in body in which metabolic alterations are mainly reproduced is the blood. Moringa oleifera, originally from India, has been scientifically assessed for various medicinal applications.

Aims and Objective: To examine the effect of M. oleifera aqueous extract taken from the leaf on total leukocyte count including its differentials (neutrophils and lymphocytes) in cadmium-treated rats.

Materials and Methods: Twenty-four adult Wistar Albino rats, weighing between 180 and 200 g were broadly divided into four groups, six animals in each group, with group I being the control. Group IV was pretreated with the extract and then cadmium chloride orally for 1 day. Data were presented as mean ± SD and p ≤ 0.05 considered significant.

Result: The results portrayed that pretreatment with Moringa leaf extract, 100 mg/kg/bw, earlier to the cadmium administration exhibited a significant increase (p ≤ 0.001) in the total leukocyte count including its differentials in comparison with the cadmium-exposed group, whereas the total WBC count was significantly decreased in group III in comparison with groups I and II. This study depicts a marked increase in the total leukocyte (T-WBC) count and its differentials in the animals pretreated with Moringa oleifera leaf extract, before the infusion of cadmium could be an indication that M. oleifera offers protection against damage to hematological parameters induced by cadmium.

Conclusion: Therefore, our study suggests that aqueous leaf extract of M. oleifera enhances the immune mechanisms to fight back foreign substances when exposed to cadmium toxicity.

Key words: Cadmium; Blood Disorders; Moringa oleifera; Leukocyte Count; Immune Mechanisms


 
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REFERENCES
1. Chatterjee PK, Vinodini NA, Amemarsoofi A, Nayanatara AK, Pai SR, Suman VB. Hypolipidemic effect of Moringa oleifera leaf extract in cadmium exposed rats. Int J Innovative Res Sci Eng Technol (IJIRSET). 2013;2(9):4718-23.
2. International Agency for Research on Cancer (IARC). Beryllium, cadmium, mercury and exposures. In: Glass Manufacturing Industry in Monographs on the Evaluation of Carcinogenic Risk San Diego, CA: Human Scientific Publications. 1993. Vol58. pp. 119-237.
3. World Health Organization. Environmental Health Criteria, 134, Cadmium. Geneva: World Health Organization, 1992. pp. 111-2.
4. Eteng MU, Onwuka FC, Akpanyung EO, Osuchukwu NC, Bassey SC, Nwankpa P. Reversal of cadmium induced toxicity following dietary supplementation with garlic, ginger and cabbage in male Wistar rats. J Natl Prod Plant Resour. 2012;2(1):169-74.
5. Vinodini NA, Chatterjee PK, Chatterjee P, Chakraborti S, Nayanatara AK, Bhat RM, et al. Protective role of aqueous leaf extract of Moringa oleifera on blood parameters in cadmium exposed adult Wistar Albino rats. Int J Curr Res Acad Rev (IJCRAR). 2015; 3(1):192-4.
6. Hounkpatin ASY, Edorh PA, Guédénon P, Alimba CG, Ogunkanmi A, Dougnon TV, et al. Haematological evaluation of Wistar rats exposed to chronic doses of cadmium, mercury and combined cadmium and mercury. Afr J Biotechnol. 2013;12(23):3731-7.
7. Adedapo AA, Mogbojuri OM, Emikpe BO. Safety evaluations of the aqueous extract of the leaves of Moringa oleifera in rats. J Med Plants Res. 2009;3(8):586-91.
8. Asomugha AL, Ezejindu DN, Asomugha RN, Anyabolu AE, Ojukwu PC. Evaluation of toxicity effect of graded doses of Moringa oleifera leaf extract on blood indices using 20 adult Wistar rats. Int J Biomed Adv Res. 2015;6(2):98-102. [DOI via Crossref]   
9. Mukherjee P. Extraction of herbal drugs. In: Quality Control on Herbal Drugs. An Approach to Evaluation of Botanicals, 1st edn. Chapter 11. New Delhi, India: Business Horizons, 2002. pp. 371-425.
10. Ghai CL. A Textbook of Practical Physiology. New Delhi, India: Jaypee Brothers Medical Publishers (P) Ltd, 2013. pp. 60-84.
11. Antai AB, Ofem OE, Ikpi DE, Ukafia S, Agiang EA. Phytochemistry and some haematological changes following oral administration of ethanolic root extract of Gongronema latifolium in rats. Niger J Physiol Sci. 2009;24(1):79-8.
12. Coles EH. Veterinary Clinical Pathology. Philadelphia, PA: WB Saunders, Co, 1986. pp. 5-87.
13. Seymore T. Bioaccumulation of Metals in Barbus marequensis from the Olifants River, Krugar National Park and Lethal Levels of Manganese to Juvenile Oreochromis mossambicusMSc Thesis, Rand, Afrikaans University: South Africa1994.
14. Lodi S, Kansala L. Antioxidant activity of Rubia cordifolia against lead toxicity. Int J Pharmaceut Sci Res. 2012;3(7):2224-32.
15. Ibiam UA, Ugwuja EI, Ejeogo C, Aja PM, Afiukwa C, Oji OU, et al. Hemoprotective and nephroprotective potentials of aqueous extract of Jussiaea nervosa leaf in cadmium exposed Albino rats. IOSR J Pharm Biol Sci (IOSR-JPBS). 2012;4(1):48-53. [DOI via Crossref]   
16. Horiguchi H, Oguma E, Kayama F. Cadmium induces anemia through interdependent progress of hemolysis, body iron accumulation, and insufficient erythropoietin production in rats. Toxicol Sci. 2011; 122(1):198-210. [DOI via Crossref]    [Pubmed]   
17. Ojo OA, Ajiboye BO, Oyinloye BE, Ojo AB. Hematological properties of Irvingia gabonensis in male adult rats. J Pharmaceut Sci Innovat (JPSI). 2014;3(5):434-5. [DOI via Crossref]   
18. Goyal BR, Agarwal BB, Goyal RK, Mehta AA. Phyto-pharmacology of Moringa oleifera Lam.ó—an overview. Nat Prod Radiance. 2007; 6(4):347-53.
19. Otitoju O, Nwamarah JU, Otitoju GTO, Okorie AU, Stevens C, Baiyeri KP. Effect of Moringa oleifera aqueous leaf extract on some haematological indices in Wistar rats. Chem Process Eng Res. 2014;18:26-30.
20. Mazumder UK, Gupta M, Chakrabarti S, Pal D. Evaluation of hematological and hepatorenal functions of methanolic extract of Moringa oleifera Lam. root treated mice. Indian J Exp Biol. 1999;37 (6):612-4.
21. Paul WE. Infectious Diseases and the Immune SystemBig Sandy, TX: Scientific American199390.
22. Swenson MJ, Reece OW. Duke’s Physiology of Domestic Animals. Ithaca, NY: Comstock Publishing Associates, 1993. pp. 221-35.
23. Adedapo AA, Adegbayibi AY, Emikpe BO. Some clinicopathological changes associated with the aqueous extract of the leaves of Phyllanthus amarus in rats. Phytother Res. 2005;19:971-6. [DOI via Crossref]    [Pubmed]   

This Article Cited By the following articles

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How to Cite this Article
Pubmed Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya. Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model. Natl J Physiol Pharm Pharmacol. 2016; 6(2): 119-122. doi:10.5455/njppp.2016.6.2709201591


Web Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya. Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model. https://www.njppp.com/?mno=202157 [Access: June 01, 2022]. doi:10.5455/njppp.2016.6.2709201591


AMA (American Medical Association) Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya. Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model. Natl J Physiol Pharm Pharmacol. 2016; 6(2): 119-122. doi:10.5455/njppp.2016.6.2709201591



Vancouver/ICMJE Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya. Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model. Natl J Physiol Pharm Pharmacol. (2016), [cited June 01, 2022]; 6(2): 119-122. doi:10.5455/njppp.2016.6.2709201591



Harvard Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya (2016) Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model. Natl J Physiol Pharm Pharmacol, 6 (2), 119-122. doi:10.5455/njppp.2016.6.2709201591



Turabian Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya. 2016. Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model. National Journal of Physiology, Pharmacy and Pharmacology, 6 (2), 119-122. doi:10.5455/njppp.2016.6.2709201591



Chicago Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya. "Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model." National Journal of Physiology, Pharmacy and Pharmacology 6 (2016), 119-122. doi:10.5455/njppp.2016.6.2709201591



MLA (The Modern Language Association) Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya. "Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model." National Journal of Physiology, Pharmacy and Pharmacology 6.2 (2016), 119-122. Print. doi:10.5455/njppp.2016.6.2709201591



APA (American Psychological Association) Style

Pratik Kumar Chatterjee, Vinodini Nithyanandamadom Anantharaya, Anish Singhal, Poulomi Chatterjee, Rashmi Kapu Shiva, Roopashree Mallya (2016) Moringa oleifera aqueous leaf extract: role on total leucocyte count and its differentials in cadmium toxicity in adult Wistar Albino rat model. National Journal of Physiology, Pharmacy and Pharmacology, 6 (2), 119-122. doi:10.5455/njppp.2016.6.2709201591