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Original Research 


Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin.

Cited by (2)

Abstract
Background: Phyllanthus niruri, Thymus vulgaris, Centella asiatica, and Apium graveolens L. have been frequently used as medical herbs for various diseases. However, the use of the herbs is not based on any evidence of their contents.

Aims and Objective: To examine their potential activity of becoming antioxidant, using in vitro and in vivo models and isolate active compound.

Materials and Methods: The antioxidant activity of the samples was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) as radical compounds. The decrease of DPPH radical was measured using spectrophotometer at 517 nm, after an incubation for 30 min. The highest antioxidant activity of the sample was then continued with in vivo model using Sprague Dawley (SD) rats. Paracetamol was used as an inducer to increase the malondialdehyde (MDA) levels in plasma, with doses of 2.5 g/kg BW. The rats were divided into four groups: negative control, dose of 50 mg/kg BW, dose of 100 mg/kg BW, and dose of 200 mg/kg BW. Isolation method was guided by antioxidant activity by using fractionation and column chromatography. The obtained compound was then characterized by 1H-nuclear magnetic resonance (NMR) and compared with the earlier study.

Result: The IC50 value of these samples were 14.21 ± 0.73; 14.41 ± 1.13; 98.66 ± 9.59; and 237.33 ± 17.26 mg/mL, respectively, and the total phenolic contents were 81.59 ± 2.85; 154.09 ± 11.61; 6.36 ± 3.99; 2.03 ± 0.78 mg/g of sample extract, respectively. The in vivo study showed that P. niruri with dose of 100 mg/kg BW and 200 mg/kg BW were able to reduce MDA levels by 90.44% and 92.64%, respectively. Meanwhile, the proton and carbon NMR spectra showed that the isolate was quercetin. The IC50 value of the isolate, being 5.85 mg/mL, was lower than that of vitamin E, which was 6.85 mg/mL.

Conclusion: The extract shows a potential effect with antioxidant activity, both in in vitro and in vivo models. Quercetin is the active compound, the antioxidant activity of which is higher than that of vitamin E.

Key words: Antioxidant; Phyllanthus niruri L.; Quercetin


 
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REFERENCES
1. Zou Y, Lu Y, Wei D. Antioxidant activity of flavonoid-rich extract of Hypericum perforatum L. in vitro. J Agric Food Chem. 2004;52 (16):5032-9. [DOI via Crossref]    [Pubmed]   
2. Hariyatmi. Kemampuan Vitamin E sebagai Antioksidan terhadap Radikal Bebas pada Lanjut Usia. MIPA, Jurusan Pendidikan Biologi FKIP UMS. Surakarta. 2004;14(1):52-60.
3. Suirta IWNM, Puspawati, Gumiati NK. Isolasi dan Identifikasi Senyawa Aktif Larvasida dari Biji Mimba (Azadirachta indika A. Juss) Terhadap Larva Nyamuk Demam Berdarah (Aedes aegypti). J Kimia. 2007;1(1):47-54.
4. Temple NJ. Antioxidants and disease: more questions than answers. Nutrition Research. 2000;20(3):449-59. [DOI via Crossref]   
5. Ueno Y, Kizaki M, Nakagiri R, Kamiya T, Sumi H, Oswa T. Dietary glutathione protects rats from diabetic nephropathy and neuropathy. J. Nutr. 2000;132(5):897-900.
6. Amrun MH, Umiyah Evi UU. Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Metanol beberapa Varian Buah Kenitu (Chrysophyllum cainito L.) dari Daerah Jember Berkala Penelelitian Hayati. 2007;13:45-50.
7. Rosen P, Tritschler HJ, Packer L. Vascular complications in diabetes: mechanisms and the influence of antioxidants In:(Eds.) Handbook of Antioxidants, 2nd edn. , New York: Marcel Dekker, 2000. pp. 511-24.
8. Da’i M, Wahyuni AS, Kusumowati ITD, Setiawan D, Dhi’fi HJ, Suhendi A, et al. The Corrrelation of Phenolic Content with Antioxidant Activity Five Types Indonesian Herbs International Seminar on Translational Research in Cancer Chemoprevention2011.
9. Paithankar VV, Raut KS, Charde RM, Vyas JV. Phyllanthus niruri: a magic herb. Res Pharm. 2011;1(4):1-9.
10. Zhang LL, Lin YM. Antioxidant tannins from Syzygium cumini fruit Afr J Biotechnol. 2009;8(10):2301-9.
11. Alfarabi M, Bintang M, Suryani, Safithri M. The Comparative ability of antioxidant activity of Piper crocatum in inhibiting fatty acid oxidation and free radical scavenging. Hayati J Biosci. 2010;17(4):201-4. [DOI via Crossref]   
12. Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem. 2003;83(4):547-50. [DOI via Crossref]   
13. Karadeniz F, Burdurlu HS, Koca N, Soyer Y. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turk J Agric For. 2004;29:297-303.
14. Waterhouse AL. Current Protocols in Food Analytical Chemistry. United States: John Wiley and Sons, 2000. pp. 11-8.
15. Sriningsih. Efek protektif pemberian ekstrak etanol herba meniran (Phyllantus niruri L.) terhadap aktivitas dan kapasitas fagositosis makrofag peritoneum tikus. Artocarpus. 2006;6(2):91-6.
16. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95 (2):351-8. [DOI via Crossref]   
17. Reynertson KA. Phytochemical Analysis of Bioactive Constituents from Edible Myrtaceae Fruit, Dissertation, The City University of New York2007. pp. 70-4.
18. Corcoran GB, Racz WJ, Smith CV, Mitchell JR. Effects of N-acetylcysteine on acetaminophen covalent binding and hepatic necrosis in mice. J Pharmacol Exp Ther. 1985;232(3):864-72.
19. Josephy PD. Molecular Toxicology. , New York: Oxford University Press, 2007. pp. 97-104.
20. Widhihastuti E. Pengukuran Aktivitas Antioksidan dengan Metode DPPH serta Korelasinya dengan Kadar Fenolik pada Lima Jenis Herba Bahan Obat Alam Indonesia. Skripsi. Fakultas Farmasi Universitas Muhammadiyah Surakarta. 2011.
21. Widyastuti N. Pengukuran Aktivitas Antioksidan dengan Metode CUPRAC, DPPH, dan FRAP serta Korelasinya dengan Fenol dan Flavonoid pada Enam Tanaman. Skripsi. Fakultas MIPA Institut Pertanian Bogor2010.
22. Da’i M, Wahyuni AS, Kusumowati ITD, Suhendi A, Azizah T. Pemanfaatan Antioksidan Alami Pada Upaya Pencegahan Komplikasi Akibat Stres Oksidatif Pada Tikus Yang Diinduksi Diabetes Melitus, Laporan Penelitian LPPM UMS2012.
23. Shivanandappa T, Harish R. Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chem. 2006;95(2): 180-5. [DOI via Crossref]   
24. Shamasundar KV, Singh B, Thakur RS, Husain A, Kiso Y, Hikino H. Antihepatotoxic principles of Phyllanthus niruri herbs. J Ethnopharmacol. 1985;14(1):41-4. [DOI via Crossref]   
25. Makoshi MS, Adanyeguh IM, Nwatu LI. Hepatoprotective effect of Phyllanthus niruri aqueous extract in acetaminophen sub-acutec exposure rabbits. J Vet Med Anim Health. 2013;5(1):8-15.
26. Foo LY, Lu Y, Molan AL, Woodfield DR, McNabb WC. The phenols and prodelphinidins of white clover flowers. Phytochemistry. 2000;54(5):539-48. [DOI via Crossref]   

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How to Cite this Article
Pubmed Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin. Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. Natl J Physiol Pharm Pharmacol. 2016; 6(1): 32-37. doi:10.5455/njppp.2015.5.0510201575


Web Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin. Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. https://www.njppp.com/?mno=203941 [Access: August 03, 2022]. doi:10.5455/njppp.2015.5.0510201575


AMA (American Medical Association) Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin. Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. Natl J Physiol Pharm Pharmacol. 2016; 6(1): 32-37. doi:10.5455/njppp.2015.5.0510201575



Vancouver/ICMJE Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin. Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. Natl J Physiol Pharm Pharmacol. (2016), [cited August 03, 2022]; 6(1): 32-37. doi:10.5455/njppp.2015.5.0510201575



Harvard Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin (2016) Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. Natl J Physiol Pharm Pharmacol, 6 (1), 32-37. doi:10.5455/njppp.2015.5.0510201575



Turabian Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin. 2016. Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. National Journal of Physiology, Pharmacy and Pharmacology, 6 (1), 32-37. doi:10.5455/njppp.2015.5.0510201575



Chicago Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin. "Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound." National Journal of Physiology, Pharmacy and Pharmacology 6 (2016), 32-37. doi:10.5455/njppp.2015.5.0510201575



MLA (The Modern Language Association) Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin. "Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound." National Journal of Physiology, Pharmacy and Pharmacology 6.1 (2016), 32-37. Print. doi:10.5455/njppp.2015.5.0510201575



APA (American Psychological Association) Style

Muhammad Da’i, Arifah Sri Wahyuni, Ika Trisharyanti DK, Tanti Azizah, Andi Suhendi, Azis Saifudin (2016) Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. National Journal of Physiology, Pharmacy and Pharmacology, 6 (1), 32-37. doi:10.5455/njppp.2015.5.0510201575