E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Da'i.

Cited by (6)

Abstract
Background: Black rice bran contains bioactive compounds; previous studies have shown the ethanol extract of black rice bran (EEBRB) can decrease levels in diabetic rats.

Aims and Objective: To elucidate the mechanism of antidiabetic effect of EEBRB.

Materials and Methods: This research was carried out by setting a few parameters for antidiabetic. The effects of inhibition of the enzyme alpha-glucosidase performed with in vitro studies using the extracted enzyme of intestines of mice. A total of 5 mL alpha-glucosidase enzyme was incubated for 5 min with EEBRB 1 mL with a serial concentration in phosphate buffer pH 7.2. Enzyme inhibitory effect was determined by measuring the concentration of formed glucose with Lane–Eynon method. The insulin level and glucose-lowering activity were determined on rats induced by alloxan 150 mg/kg and treated with EEBRB. Treated rats for 28 days were sacrificed and were prepared for histopathological observation of pancreas.

Result: EEBRB was able to inhibit the activity of the enzyme a-glucosidase on glucose with IC50 values of 121 ± 12.3 mg%. The EEBRB extract dose of 100 mg/kg was able to decrease blood sugar levels to 151 ± 38.58 mg/dL. The level of insulin in diabetic rats administered by EEBRB dose of 50, 100, and 200 mg/kg was obtained 6.52 ± 5.94, 11.5 ± 3.5, and 15.20 ± 9.5 ng/mL, respectively. Meanwhile, histopathological observations showed an improvement on the pancreatic beta cells.

Conclusion: Antidiabetic mechanism of EEBRB is associated with increased insulin levels because of regeneration of pancreatic beta cells in diabetic rats.

Key words: Black Rice Bran; Antidiabetic; Insulin Level; Pancreatic Beta Cells


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Arifah Sri Wahyuni
Articles by Rima Munawaroh
Articles by Muhammad Da'i
on Google
on Google Scholar


REFERENCES
1. Zhang M, Zhang RF, Zhang FX, Liu RH. Phenolic profiles and antioxidant activity of black rice bran of different commercially available varieties. J Agric Food Chem. 2010;58(13):7580-7. [DOI via Crossref]    [Pubmed]   
2. Ichikawa H, Ichiyanagi T, Xu B, Yoshii Y, Nakajima M, Konishi T. Antioxidant activity of anthocyanin extract from purple black rice. J Med Food. 2001;4(4):211-8. [DOI via Crossref]    [Pubmed]   
3. Raghvendra V, Shakya A, Hedaytullah M, Arya GS, Mishra A, et al. Chemical and potential aspects of anthocyanins-a water-soluble vacuolar flavonoid pigments: a review. IJPSRR. 2011;6(1):28-33.
4. Tsuda TK, Shiga K, Ohsima S, Kawakishi T. Inhibition of lipid peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L Biochem Pharmacol. 1996;52(7):1033-10.
5. Inaguma T, Han J, Isoda H Improvement of insulin resistance by cyanidin-3-glucoside, anthocyanin from black beans through the up-regulation of GLUT4 gene expression BMC Proc. 2011;5(Suppl 8): 21 Available athttp://www.pubmedcentral.nih.gov/articlerender.fcgi? artid=3284935&tool=pmcentrez&rendertype=abstract. Last accessed on October 26, 2015.
6. Jang H-H, Park M-Y, Kim H-W, Lee Y-M, Hwang K-A, Park J-H, et al. Black rice (Oryza sativa L.) extract attenuates hepatic steatosis in C57BL/6 J mice fed a high-fat diet via fatty acid oxidation Nutr Metab (Lond). 2012;9(1):27 Available athttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3366884&tool=pmcentrez&rendertype=abstract.
7. Wahyuni AS, Munawwaroh R. Economic value added of black rice bran as antidiabetic:Pharmacology and Phytochemistry Studies, 2014.
8. Tapas AR, Sakarkar D, Kakde RB. Flavonoids as nutraceuticals: a review. Trop J Pharm Res. 2008;7(3):1089-99. [DOI via Crossref]   
9. Mataputun PS, Rorong JA, Pontoh J. Alpha Glucosidase inhibitory activity of extract of bark of Matoa (Pometia Spp) as antihyperglicemic agent. J MIPA USRAT. 2013;2(2):119-23.
10. Moradi-Afrapoli F, Asghari B, Saeidnia S, Ajani Y, Mirjani M, Malmir M, et al. In vitro alpha-glucosidase inhibitory activity of phenolic constituents from aerial parts of Polygonum hyrcanicum. DARU J Pharm Sci. 2012;20(1):37. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
11. Borges de Melo E, da Silveira Gomes A, Carvalho I. a- and b-Glucosidase inhibitors: chemical structure and biological activity. Tetrahedron. 2006;62(44):10277-302. [DOI via Crossref]   
12. Akkarachiyasit S, Charoenlertkul P, Yibchok-Anun S, Adisakwattana S. Inhibitory activities of cyanidin and its glycosides and synergistic effect with acarbose against intestinal a-glucosidase and pancreatic a-amylase Int J Mol Sci. 2010;11(9):3387-96. Available at http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2956102&tool=pmcentrez&rendertype=abstract.
13. Sasaki R, Nishimura N, Hoshino H, Isa Y, Kadowaki M, Ichi T, et al. Cyanidin 3-glucoside ameliorates hyperglycemia and insulin sensitivity due to downregulation of retinol binding protein 4 expression in diabetic mice Biochem Pharmacol. 2007;74(11):1619-27. Available at http://www.sciencedirect.com/science/article/pii/S000629520700545X.
14. Guo H, Ling W, Wang Q, Liu C, Hu Y, Xia M, et al. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats. Plant Foods Hum Nutr. 2007;62(1):1-6. [DOI via Crossref]    [Pubmed]   
15. Brahmachari G. Six bio-flavonoids with promising antidiabetic potentials: a critical survey. Res Signpost Chall Scope Nat Prod Med Chem. 2011;37661(2):187-212.

This Article Cited By the following articles


2022; (): 195.

1
 
Antioxidant activity and inhibition of α-amylase and α-glucosidase in fermented black rice bran-based analog rice
AIMSAGRI 2022; 7(1): 61.

2
 
Genetic and phytochemical analysis of Indonesian black rice cultivars
J. Crop Sci. Biotechnol. 2021; (): .

3
 
Biological Functions and Activities of Rice Bran as a Functional Ingredient: A Review
Nutr Metab Insights 2021; 14(): 117863882110585.

4
 
Rice - not just a staple food: A comprehensive review on its phytochemicals and therapeutic potential
Trends in Food Science & Technology 2020; 97(): 265.

5
 
The Genetic Basis and Nutritional Benefits of Pigmented Rice Grain
Front. Genet. 2020; 11(): .

6
 
How to Cite this Article
Pubmed Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol. 2016; 6(2): 106-110. doi:10.5455/njppp.2015.5.1111201590


Web Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. https://www.njppp.com/?mno=207825 [Access: June 01, 2022]. doi:10.5455/njppp.2015.5.1111201590


AMA (American Medical Association) Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol. 2016; 6(2): 106-110. doi:10.5455/njppp.2015.5.1111201590



Vancouver/ICMJE Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol. (2016), [cited June 01, 2022]; 6(2): 106-110. doi:10.5455/njppp.2015.5.1111201590



Harvard Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai (2016) Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. Natl J Physiol Pharm Pharmacol, 6 (2), 106-110. doi:10.5455/njppp.2015.5.1111201590



Turabian Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. 2016. Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (2), 106-110. doi:10.5455/njppp.2015.5.1111201590



Chicago Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. "Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats." National Journal of Physiology, Pharmacy and Pharmacology 6 (2016), 106-110. doi:10.5455/njppp.2015.5.1111201590



MLA (The Modern Language Association) Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai. "Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats." National Journal of Physiology, Pharmacy and Pharmacology 6.2 (2016), 106-110. Print. doi:10.5455/njppp.2015.5.1111201590



APA (American Psychological Association) Style

Arifah Sri Wahyuni, Rima Munawaroh, Muhammad Dai (2016) Antidiabetic mechanism of ethanol extract of black rice bran on diabetic rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (2), 106-110. doi:10.5455/njppp.2015.5.1111201590