E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research

Online Publishing Date:
19 / 05 / 2017

 


Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante.


Abstract
Background: There is an increasing incidence of liver failure and lack of effective drugs for liver diseases.

Aim and Objective: This study identified the phytochemicals present in aqueous and ethanolic Bixa orellana L. leaf extracts and investigated the hepatoprotective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. Specifically, the study compared serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and examined liver tissues histopathology.

Materials and Methods: Aqueous and ethanolic B. orellana L. leaf extracts were prepared and screened for phytochemical contents. An oral dose of 500 mg/kg body weight was identified from toxicity test and administered to albino mice. Microscopic examination of the liver tissues assessed the extent of hepatic injury. Serum AST and ALT levels were compared using one-way analysis of variance with Bonferroni post hoc analysis at 5% level of significance using Stata/SE V12.0 software.

Results: B. orellana L. leaves contained alkaloids, anthraquinones, sugars, and tannins. Aqueous and ethanolic leaf extracts of B. orellana L. did not show any toxicity up to 2000 mg/kg body weight oral dose in mice. Pre-treatment for 7 days before CCl4 administration significantly prevented elevation of serum AST and ALT levels with histopathologic findings showing a protective effect on the hepatocytes.

Conclusion: B. orellana L. leaves have potent hepatoprotective activity against oxidative damage.

Key words: Bixa orellana L.; Leaf Extract; Carbon Tetrachloride; Hepatotoxicity; Liver Enzymes; Oxidative Damage


 
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Pubmed Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol. 2017; 7(9): 972-976. doi:10.5455/njppp.2017.7.0412011052017


Web Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. https://www.njppp.com/?mno=264257 [Access: March 14, 2024]. doi:10.5455/njppp.2017.7.0412011052017


AMA (American Medical Association) Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol. 2017; 7(9): 972-976. doi:10.5455/njppp.2017.7.0412011052017



Vancouver/ICMJE Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol. (2017), [cited March 14, 2024]; 7(9): 972-976. doi:10.5455/njppp.2017.7.0412011052017



Harvard Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante (2017) Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. Natl J Physiol Pharm Pharmacol, 7 (9), 972-976. doi:10.5455/njppp.2017.7.0412011052017



Turabian Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante. 2017. Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. National Journal of Physiology, Pharmacy and Pharmacology, 7 (9), 972-976. doi:10.5455/njppp.2017.7.0412011052017



Chicago Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante. "Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity." National Journal of Physiology, Pharmacy and Pharmacology 7 (2017), 972-976. doi:10.5455/njppp.2017.7.0412011052017



MLA (The Modern Language Association) Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante. "Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity." National Journal of Physiology, Pharmacy and Pharmacology 7.9 (2017), 972-976. Print. doi:10.5455/njppp.2017.7.0412011052017



APA (American Psychological Association) Style

Cristine P Lopez, Derick Erl P Sumalapao, Nelson R Villarante (2017) Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity. National Journal of Physiology, Pharmacy and Pharmacology, 7 (9), 972-976. doi:10.5455/njppp.2017.7.0412011052017