Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study

Arijit Sarkar; Kalidas Rit; Shuvankar Mukherjee

doi:10.5455/njppp.2023.13.06294202328062023

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Original Research

Natl J Physiol Pharm Pharmacol. 2024; 14(2): 213-217

doi: 10.5455/njppp.2023.13.06294202328062023

Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study

Arijit Sarkar, Kalidas Rit, Shuvankar Mukherjee.

Abstract
Background: In December 2019 in Wuhan, China, a new coronavirus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appeared. Gradually, the virus started spreading around the world, which was life-threatening.

Aims and Objectives: The study’s objective was to find out the positivity rate of infection, the category of patients included in the study, the percentage of positive and negative cases, comparison of rapid antigen detection in relation to real-time reverse transcription–polymerase chain reaction (RT-PCR) test, and rapid antigen detection test’s sensitivity and specificity.

Materials and Methods: Eight hundred sixty-five patients’ samples and nasopharyngeal swabs were collected within 3 months as routine samples. Two hundred thirty-two samples were compared by both rapid antigen test (RAT) and RT-PCR. To rule out false negatives, those who tested negative for COVID-19 using a RAT underwent testing by RT-PCR. To rule out false positives, a small number of patients who tested positive for COVID-19 by RAT also underwent validation by RT-PCR test.

Results: Two hundred thirty-two samples were obtained for rapid antigen detection, then RT-PCR testing. Both the results were compared. According to the findings, the positivity rate of COVID-19 infection was 0.86%. Two patients (0.86%) tested positive (either by one or both methods) and the number of patients who tested negative was 230 (99.14%). RAT sensitivity and specificity were 50% and 100%, respectively, compared to gold standard RT-PCR. The positive predictive value was 100% and the negative predictive value was 99%.

Conclusion: We conclude that antigen detection methods are less sensitive than real-time RT-PCR but they can be used for COVID-19 mass screening and can be used as a quick diagnostic tool in remote locations without molecular testing facilities.

Key words: COVID-19; Rapid Antigen Testing; RT-PCR; SARS-CoV-2

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Pubmed Style

Sarkar A, Rit K, Mukherjee S. Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study. Natl J Physiol Pharm Pharmacol. 2024; 14(2): 213-217. doi:10.5455/njppp.2023.13.06294202328062023

Web Style

Sarkar A, Rit K, Mukherjee S. Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study. https://www.njppp.com/?mno=155330 [Access: January 18, 2025]. doi:10.5455/njppp.2023.13.06294202328062023

AMA (American Medical Association) Style

Sarkar A, Rit K, Mukherjee S. Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study. Natl J Physiol Pharm Pharmacol. 2024; 14(2): 213-217. doi:10.5455/njppp.2023.13.06294202328062023

Vancouver/ICMJE Style

Sarkar A, Rit K, Mukherjee S. Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study. Natl J Physiol Pharm Pharmacol. (2024), [cited January 18, 2025]; 14(2): 213-217. doi:10.5455/njppp.2023.13.06294202328062023

Harvard Style

Sarkar, A., Rit, . K. & Mukherjee, . S. (2024) Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study. Natl J Physiol Pharm Pharmacol, 14 (2), 213-217. doi:10.5455/njppp.2023.13.06294202328062023

Turabian Style

Sarkar, Arijit, Kalidas Rit, and Shuvankar Mukherjee. 2024. Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study. National Journal of Physiology, Pharmacy and Pharmacology, 14 (2), 213-217. doi:10.5455/njppp.2023.13.06294202328062023

Chicago Style

Sarkar, Arijit, Kalidas Rit, and Shuvankar Mukherjee. "Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study." National Journal of Physiology, Pharmacy and Pharmacology 14 (2024), 213-217. doi:10.5455/njppp.2023.13.06294202328062023

MLA (The Modern Language Association) Style

Sarkar, Arijit, Kalidas Rit, and Shuvankar Mukherjee. "Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study." National Journal of Physiology, Pharmacy and Pharmacology 14.2 (2024), 213-217. Print. doi:10.5455/njppp.2023.13.06294202328062023

APA (American Psychological Association) Style

Sarkar, A., Rit, . K. & Mukherjee, . S. (2024) Diagnosis of COVID-19 obtained by rapid antigen test and reverse transcription–polymerase chain reaction in a medical college in Eastern India-a record-based observational descriptive study. National Journal of Physiology, Pharmacy and Pharmacology, 14 (2), 213-217. doi:10.5455/njppp.2023.13.06294202328062023

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