Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital

Sritanu Jana; Debasis Mukhopadhyay; Sunita Bagdi; Arindam De; Swapan Pathak

doi:10.5455/njppp.2023.13.12606202205012022

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17 / 01 / 2023

Natl J Physiol Pharm Pharmacol. 2023; 13(2023, Vol: 13, Issue: 8): 1634-1638

doi: 10.5455/njppp.2023.13.12606202205012022

Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital

Sritanu Jana, Debasis Mukhopadhyay, Sunita Bagdi, Arindam De, Swapan Pathak.

Abstract
Background: Pulmonary tuberculosis (TB) is common clinical presentation in India, but, nowadays, extrapulmonary TB (EPTB) is also a clinical problem. At present, no single test is available for diagnosing EPTB which is rapid, cost-effective, and confirmatory. Many studies were done to diagnose and treat the pulmonary TB, though more efforts are needed for diagnosis of EPTB.

Aims and Objectives: The aim of this study is to find out any test or a series of tests that reduce the diagnostic dilemma of cervical lymph node TB.

Materials and Methods: A total of 170 patients with cervical lymphadenopathy were enrolled in the pathology department between July 1, 2021, and December 31, 2021. It was a descriptive study and of cross-sectional design. The Institutional Ethics Committee approval was received (No. BSMC/IEC/101 dt. January 15, 2021). Suspected malignancy cases were excluded from the study. Fine-needle aspiration (FNA) was done for all these patients and stained with Leishman-Geimsa, Papaniculaou, and Zeihl-Neelsen (ZN) stain. After FNA, all samples were sent for cartridge based nucleic acid amplification test.

Results: After FNA cytology, ZN stain was done and only 9.2% of acid–fast bacilli (AFB) positive cases were found. Granuloma was found in 27.6% of cases. After nucleic acid amplification test (NAAT) study, it was found that 36.8% patients were positive. Total number of lymph node considered to be positive for TB (NAAT positive and/or ZN stain positive and/or presence of granuloma) is 85 (52.1%). As per conventional method for diagnosis of lymph node TB (presence of granuloma and/or AFB positive in ZN stain), number of cases were 50 (30.7%). NAAT test was significantly more positive in respect of ZN staining but not significantly positive in respect of presence of granuloma.

Conclusion: For diagnosis of cervical lymph node TB, all three methods (NAAT, ZN stain, and Leishman-Giemsa stain) should be used simultaneously.

Key words: Cartridge-Based Nucleic Acid Amplification Test; Extrapulmonary Tuberculosis; Cervical Lymphadenopathy

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Pubmed Style

Jana S, Mukhopadhyay D, Bagdi S, De A, Pathak S. Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital. Natl J Physiol Pharm Pharmacol. 2023; 13(2023, Vol: 13, Issue: 8): 1634-1638. doi:10.5455/njppp.2023.13.12606202205012022

Web Style

Jana S, Mukhopadhyay D, Bagdi S, De A, Pathak S. Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital. https://www.njppp.com/?mno=103991 [Access: March 14, 2024]. doi:10.5455/njppp.2023.13.12606202205012022

AMA (American Medical Association) Style

Jana S, Mukhopadhyay D, Bagdi S, De A, Pathak S. Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital. Natl J Physiol Pharm Pharmacol. 2023; 13(2023, Vol: 13, Issue: 8): 1634-1638. doi:10.5455/njppp.2023.13.12606202205012022

Vancouver/ICMJE Style

Jana S, Mukhopadhyay D, Bagdi S, De A, Pathak S. Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital. Natl J Physiol Pharm Pharmacol. (2023), [cited March 14, 2024]; 13(2023, Vol: 13, Issue: 8): 1634-1638. doi:10.5455/njppp.2023.13.12606202205012022

Harvard Style

Jana, S., Mukhopadhyay, . D., Bagdi, . S., De, . A. & Pathak, . S. (2023) Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital. Natl J Physiol Pharm Pharmacol, 13 (2023, Vol: 13, Issue: 8), 1634-1638. doi:10.5455/njppp.2023.13.12606202205012022

Turabian Style

Jana, Sritanu, Debasis Mukhopadhyay, Sunita Bagdi, Arindam De, and Swapan Pathak. 2023. Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital. National Journal of Physiology, Pharmacy and Pharmacology, 13 (2023, Vol: 13, Issue: 8), 1634-1638. doi:10.5455/njppp.2023.13.12606202205012022

Chicago Style

Jana, Sritanu, Debasis Mukhopadhyay, Sunita Bagdi, Arindam De, and Swapan Pathak. "Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital." National Journal of Physiology, Pharmacy and Pharmacology 13 (2023), 1634-1638. doi:10.5455/njppp.2023.13.12606202205012022

MLA (The Modern Language Association) Style

Jana, Sritanu, Debasis Mukhopadhyay, Sunita Bagdi, Arindam De, and Swapan Pathak. "Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital." National Journal of Physiology, Pharmacy and Pharmacology 13.2023, Vol: 13, Issue: 8 (2023), 1634-1638. Print. doi:10.5455/njppp.2023.13.12606202205012022

APA (American Psychological Association) Style

Jana, S., Mukhopadhyay, . D., Bagdi, . S., De, . A. & Pathak, . S. (2023) Is nucleic acid amplification test sufficient enough for reducing the diagnostic dilemma of cervical lymph node tuberculosis? A descriptive study in a tertiary care hospital. National Journal of Physiology, Pharmacy and Pharmacology, 13 (2023, Vol: 13, Issue: 8), 1634-1638. doi:10.5455/njppp.2023.13.12606202205012022

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