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Original Research 


Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole

Jeneth Berlin Raj, Ramakrishnan Kalaivani.

Cited by (3)

Abstract
Background: In developing countries, helminthic infections pose a great threat to public health. Traditional medical remedies using plant products as antiparasitic agents have been in trail throughout the world accounting to limited availability and affordability of modern medicine. More recently, the medicinal plant Costus pict us D. Don has been extensively studied by researchers because of its diverse biochemical properties.

Objective: This study was designed to analyze the plant for its primary constituents and to evaluate the anthelmintic property of methanolic and aqueous extract of leaves and rhizomes of C. pictus D. Don in different concentrations and compare it against the standard drug albendazole using earthworm Pheretima posthuma as an animal model.

Materials and Methods: Anthelmintic property was tested by exposing the worms (18 groups with 3 worms each) one after another to different concentrations (25 mg/ml, 50 mg/ml, 75 mg/ml, and 100 mg/ml) of methanolic and aqueous extracts of leaves and rhizomes of C. pictus D. Don. The absence of movement even after dipping the worm in warm water was noted as time of paralysis, and death was noted when its body color became pale.

Results: The results of our study showed both the methanolic and aqueous extract of leaves and rhizomes in all concentrations were toxic to the worms. However, methanolic extract of rhizomes at a concentration of 100 mg/ml showed more potency when compared to albendazole (20 mg/ml).

Conclusions: Methanolic extracts of leaves and rhizomes of C. pictus D. Don have potent dose-dependent anthelmintic effect when compared to their aqueous extract.

Key words: Costus pictus D. Don; Anthelmintic Property; Pheretima Posthuma; Albendazole


 
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REFERENCES
1. WHO. Soil-Transmitted Helminth Infections. 2014. Available from: http://www.who.int/mediacentre/actsheets/s366/en updated April 2014. [Last accessed on 2016 Feb 28].
2. Hotez PJ, Brindley PJ, Bethony JM, King CH, Pearce EJ, Jacobson J. Helminth infections: The great neglected tropical diseases. J Clin Invest. 2008;118(4):1311-21. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
3. Mali RG, Mehta AA. A review on anthelmintic plants. Nat Prod Radiance. 2008;7(5):466-75.
4. Sollmann T. Anthelmintics: Their efficiency as tested on earthworms. J Pharmacol Exp Ther. 1918;12:129-70.
5. Aswar M, Aswar U, Watkar B, Vyas M, Wagh A, Gujar KN. Anthelmintic activity of Ficus benghalensis. Int J Green Pharm. 2008;2:170-2. [DOI via Crossref]   
6. Srivastava S, Singh P, Jha KK, Mishra G, Srinivastava S, Khosa RL. Anthelmintic activity of aerial parts of Costus speciosus. Int J Green Pharm. 2011;5:325-8. [DOI via Crossref]   
7. Hariprasad P, Ramakrishnan. Phytochemical screening and pharmacognostical evaluation of Rumex Vesicarius L. Int J Pharmtech Res. 2011;3(2):1078-82.
8. Bhargava S, Dhabhai K, Batra A, Sharma A, Malhotra B. Zingiber officinale: Chemical and Phytochemical screening and evaluation of its antimicrobial activites. J Chem Pharm Res. 2012;4(1):360-4.
9. Thomas S, Devi BS. Phytochemical and in vitro anthelmintic studies of hydro-alcoholic extract of Costus pictus D Don. Int J Pharm Pharm Sci. 2013;5(3):639-41.
10. Williams AR, Ropiak HM, Fryganas C, Desrues O, Mueller-Harvey I, Thamsborg SM. Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum. Parasit Vectors. 2014;7:518. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
11. Stepek G, Buttle DJ, Duce IR, Lowe A, Behnke JM. Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro. Parasitology. 2005;130:203-11. [DOI via Crossref]    [Pubmed]   

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How to Cite this Article
Pubmed Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani. Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. Natl J Physiol Pharm Pharmacol. 2016; 6(5): 438-441. doi:10.5455/njppp.2016.6.0205423032016


Web Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani. Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. https://www.njppp.com/?mno=220253 [Access: January 03, 2023]. doi:10.5455/njppp.2016.6.0205423032016


AMA (American Medical Association) Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani. Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. Natl J Physiol Pharm Pharmacol. 2016; 6(5): 438-441. doi:10.5455/njppp.2016.6.0205423032016



Vancouver/ICMJE Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani. Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. Natl J Physiol Pharm Pharmacol. (2016), [cited January 03, 2023]; 6(5): 438-441. doi:10.5455/njppp.2016.6.0205423032016



Harvard Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani (2016) Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. Natl J Physiol Pharm Pharmacol, 6 (5), 438-441. doi:10.5455/njppp.2016.6.0205423032016



Turabian Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani. 2016. Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. National Journal of Physiology, Pharmacy and Pharmacology, 6 (5), 438-441. doi:10.5455/njppp.2016.6.0205423032016



Chicago Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani. "Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole." National Journal of Physiology, Pharmacy and Pharmacology 6 (2016), 438-441. doi:10.5455/njppp.2016.6.0205423032016



MLA (The Modern Language Association) Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani. "Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole." National Journal of Physiology, Pharmacy and Pharmacology 6.5 (2016), 438-441. Print. doi:10.5455/njppp.2016.6.0205423032016



APA (American Psychological Association) Style

Jeneth Berlin Raj, Ramakrishnan Kalaivani (2016) Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. National Journal of Physiology, Pharmacy and Pharmacology, 6 (5), 438-441. doi:10.5455/njppp.2016.6.0205423032016