E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky.

Cited by (2)

Abstract
Background: Breast cancer is the most frequent malignant tumor in women, and its metastatic state represents the second cause of mortality. To treat cancer, we must prevent tumor cell proliferation and angiogenesis. The usage of herbal medication for treatment of some diseases as diabetes is increased nowadays for their wide safety margin.

Aims and Objectives: Therefore, the current study was designed to examine the protective effect of ginger extract (GE) and cinnamon extract (CE) in controlling the mammary gland incidence in female Wistar rats.

Materials and Methods: A total of 75 female virgin Wistar rats were allocated into 5 groups. Control group without any treatment; mammary gland group administered 7, 12-dimethyl-benz[a]anthracene at a dose of 20 mg/kg orally in corn oil. Rats in 3-5 served as tumor groups and received GE for group 3 (0.125% in water), CE for group 4 (100 mg/kg), and a mixture of GE plus CE for group 5. GE and CE were administered 2 weeks before cancer induction and continued for 4 months. Serum levels of breast cancer biomarker (CA125) and oxidative stress biomarkers were measured. Tissues of tumors were examined at molecular and histopathological levels.

Results: There was a significant increase in the serum levels of CA125 in untreated mammary gland tumor group when compared with untreated healthy rats. There was a significant decrease in the serum levels of glutathione (GSH) peroxidase, catalase, superoxide dismutase, and GSH reductase in the untreated mammary gland tumor groups compared to control healthy rats. Administration of ginger and CE normalized the decrease in antioxidants levels. Induction of mammary gland tumor upregulated the genes associated with tumor incidences such as GST-P, CYP1A1, CYP1B1, and vascular endothelial growth factor-receptor 1 and administration of both herbal plants normalized these changes. Coadministration of GE and CE-induced additive inhibitory effect on genes upregulated due to tumor incidence. Bax expression was downregulated in tumor group and increased after GE and CE expression either alone or in combination. At histopathological levels, breast cancer group showed adenocarcinoma that is decreased with plants extracts administration when compared with breast cancer and control group.

Conclusion: Results suggest that extracts of ginger and cinnamon have anticarcinogenic protective activities and support the hypothesis that these plants help in the prevention of mammary cancer by controlling the expression of carcinogenesis-associated genes.

Key words: Herbal Medication; Carcinogenic Activity; Rats


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed Mohamed Soliman
Articles by Alshaimaa Mohammed Said Elfeky
on Google
on Google Scholar


REFERENCES
1. Kelsey JL, Horn-Ross PL. Breast cancer: Magnitude of the problem and descriptive epidemiology. Epidemiol Rev. 1993;15(1):7-16.
2. Mc Pherson K, Steel CM, Dixon JM. ABC of breast diseases. Breast cancer-epidemiology, risk factors, and genetics. BMJ. 2000;321(7261):624-8. https://doi.org/10.1136/bmj.321.7261.624
3. Marnett LJ. Oxyradicals and DNA damage. Carcinogenesis. 2000;21(3):361-70. https://doi.org/10.1093/carcin/21.3.361 [Pubmed]   
4. Hristozov D, Gadjeva V, Vlaykova T, Dimitrov G. Evaluation of oxidative stress in patients with cancer. Arch Physiol Biochem. 2001;109(4):331-6. https://doi.org/10.1076/apab.109.4.331.4248 [Pubmed]   
5. Bishayee A, Oinam S, Basu M, Chatterjee M. Vanadium chemoprevention of 7,12-dimethylbenz(a)anthracene-induced rat mammary carcinogenesis: Probable involvement of representative hepatic phase I and II xenobiotic metabolizing enzymes. Breast Cancer Res Treat. 2000;63(2):133-45. https://doi.org/10.1023/A:1006476003685 [Pubmed]   
6. Cotgreave IA, Moldéus P, Orrenius S. Host biochemical defense mechanisms against prooxidants. Annu Rev Pharmacol Toxicol. 1988;28:189-212. https://doi.org/10.1146/annurev.pa.28.040188.001201 [Pubmed]   
7. Mittal A, Pathania V, Agrawala PK, Prasad J, Singh S, Goel HC. Influence of Sinopodophyllum hexandrum on endogenous antioxidant defence system in mice: Possible role in radioprotection. J Ethnopharmacol. 2001;76(3):253-62. https://doi.org/10.1016/S0378-8741(01)00243-4
8. Prakash J, Gupta SK. Chemopreventive activity of Ocimum sanctum seed oil. J Ethnopharmacol. 2000;72(1-2):29-34.
9. Vijayavel K, Anbuselvam C, Balasubramanian MP. Free radical scavenging activity of the marine mangrove Rhizophora apiculata bark extract with reference to naphthalene induced mitochondrial dysfunction. Chem Biol Interact. 2006;163(1-2):170-5.
10. Park EJ, Pezzuto JM. Botanicals in cancer chemoprevention. Cancer Metastasis Rev. 2002;21(3-4):231-55.
11. Anderson RA, Broadhurst CL, Polansky MM, Schmidt WF, Khan A, Flanagan VP, et al. Isolation and characterization of polyphenol type-A polymers from cinnamon with insulin-like biological activity. J Agric Food Chem. 2004;52(1):65-70. https://doi.org/10.1021/jf034916b [Pubmed]   
12. Murcia MA, Egea I, Romojaro F, Parras P, Jiménez AM, Martínez-Tomé M. Antioxidant evaluation in dessert spices compared with common food additives. Influence of irradiation procedure. J Agric Food Chem. 2004;52(7):1872-81. https://doi.org/10.1021/jf0303114 [Pubmed]   
13. Nagasawa H, Watanabe K, Inatomi H. Effects of bitter melon (Momordica charantia l.) or ginger rhizome (Zingiber offifinale rosc) on spontaneous mammary tumorigenesis in SHN mice. Am J Chin Med. 2002;30(2-3):195-205.
14. Madkor HR, Mansour SW, Khalil MA. Antiangiogenic activities of cinnamon, black and green tea extracts on experimentally induced breast cancer in rats. Asian J Biochem. 2012;7(4):206-17. https://doi.org/10.3923/ajb.2012.206.217
15. Soliman MM, Baiomy AA, Yassin MH. Molecular and histopathological study on the ameliorative effects of curcumin against lead acetate-induced hepatotoxicity and nephrototoxicity in Wistar rats. Biol Trace Elem Res. 2015;167(1):91-102. https://doi.org/10.1007/s12011-015-0280-0 [Pubmed]   
16. Bancroft JD, Gamble M. Theory and Practice of Histological Techniques. 6th ed. Philadelphia, PA: Churchill Livingstone, Elsevier; 2008. p. 126-7.
17. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics, 2007. CA. Cancer J Clin. 2008;57(1):43-66. https://doi.org/10.3322/canjclin.57.1.43
18. Cassileth BR, Lusk EJ, Strouse TB, Bodenheimer BJ. Contemporary unorthodox treatments in cancer medicine. A study of patients, treatments, and practitioners. Ann Intern Med. 1984;101(1):105-12. https://doi.org/10.7326/0003-4819-101-1-105 [Pubmed]   
19. Cassileth BR. Complementary and alternative cancer medicine. J Clin Oncol. 1999;17 11 Suppl:44-52.
20. Di Paola RS, Zhang H, Lambert GH, Meeker R, Licitra E, Rafi MM, et al. Clinical and biologic activity of an estrogenic herbal combination (PC-SPES) in prostate cancer. N Engl J Med. 1998;339(12):785-91. https://doi.org/10.1056/NEJM199809173391201 [Pubmed]   
21. Buchanan DR, White JD, O'Mara AM, Kelaghan JW, Smith WB, Minasian LM. Research-design issues in cancer-symptom-management trials using complementary and alternative medicine: Lessons from the National Cancer Institute Community Clinical Oncology Program experience. J Clin Oncol. 2005;23(27):6682-9. https://doi.org/10.1200/JCO.2005.10.728 [Pubmed]   
22. Russo J, Russo IH. Experimentally induced mammary tumors in rats. Breast Cancer Res Treat. 1996;39(1):7-20. https://doi.org/10.1007/BF01806074 [Pubmed]   
23. Russo J, Russo IH. Atlas and histologic classification of tumors of the rat mammary gland. J Mammary Gland Biol Neoplasia. 2000;5(2):187-200. https://doi.org/10.1023/A:1026443305758 [Pubmed]   
24. Costa I, Solanas M, Escrich E. Histopathologic characterization of mammary neoplastic lesions induced with 7,12-dimethyl benz(alpha)anthracene in the rat: A com parative analysis with human breast tumours. Arch Pathol Lab Med. 2002;126(8):915-27.
25. Vinothini G, Murugan RS, Nagini S. Evaluation of molecular markers in a rat model of mammary carcinogenesis. Oncol Res. 2009;17(25):483-93. https://doi.org/10.3727/096504009789735459 [Pubmed]   
26. Clerkin JS, Naughton R, Quiney C, Cotter TG. Mechanisms of ROS modulated cell survival during carcinogenesis. Cancer Lett. 2008;266(1):30-6. https://doi.org/10.1016/j.canlet.2008.02.029 [Pubmed]   
27. Kumaraguruparan R, Seshagiri PB, Hara Y, Nagini S. Chemoprevention of rat mammary carcinogenesis by black tea polyphenols: Modulation of xenobiotic-metabolizing enzymes, oxidative stress, cell proliferation, apoptosis, and angiogenesis. Mol Carcinog. 2007;46(9):797-806. https://doi.org/10.1002/mc.20309 [Pubmed]   
28. Wu Y, Hooper AT, Zhong Z, Witte L, Bohlen P, Rafii S, et al. The vascular endothelial growth factor receptor (VEGFR-1) supports growth and survival of human breast carcinoma. Int J Cancer. 2006;119(7):1519-29. https://doi.org/10.1002/ijc.21865 [Pubmed]   
29. Schoene NW, Kelly MA, Polansky MM, Anderson RA. Water-soluble polymeric polyphenols from cinnamon inhibit proliferation and alter cell cycle distribution patterns of hematologic tumor cell lines. Cancer Lett. 2005;230(1):134-40. https://doi.org/10.1016/j.canlet.2004.12.039 [Pubmed]   
30. Bergers G, Benjamin LE. Tumorigenesis and the angiogenic switch. Nat Rev Cancer. 2003;3(6):401-10. https://doi.org/10.1038/nrc1093 [Pubmed]   
31. Ahmed RS, Seth V, Banerjee BD. Influence of dietary ginger (Zingiber officinales Rosc) on antioxidant defense system in rat: Comparison with ascorbic acid. Indian J Exp Biol. 2000;38(6):604-6.
32. Banerjee S, Bueso-Ramos C, Aggarwal BB Suppression of 7,12-dimethylbenz(a)anthracene-induced mammary carcinogenesis in rats by resveratrol: Role of nuclear factor-kappaB, cyclooxygenase 2, and matrix metalloprotease 9. Cancer Res. 2002;62(17):4945-54.
33. Grzanna R, Lindmark L, Frondoza CG. Ginger – An herbal medicinal product with broad anti-inflammatory actions. J Med Food. 2005;8(2):125-32. https://doi.org/10.1089/jmf.2005.8.125 [Pubmed]   
34. Shukla Y, Singh M. Cancer preventive properties of ginger: A brief review. Food Chem Toxicol. 2007;45(5):683-90. https://doi.org/10.1016/j.fct.2006.11.002 [Pubmed]   
35. Aktan F, Henness S, Tran VH, Duke CC, Roufogalis BD, Ammit AJ. Gingerol metabolite and a synthetic analogue Capsarol inhibit macrophage NF-kappaB-mediated iNOS gene expression and enzyme activity. Planta Med. 2006;72(8):727-34. https://doi.org/10.1055/s-2006-931588 [Pubmed]   
36. Kim EC, Min JK, Kim TY, Lee SJ, Yang HO, Han S, et al. [6]-Gingerol, a pungent ingredient of ginger, inhibits angiogenesis in vitro and in vivo. Biochem Biophys Res Commun. 2005;335(2):300-8. https://doi.org/10.1016/j.bbrc.2005.07.076 [Pubmed]   
37. Elkady AI, Abuzinadah OA, Baeshen NA, Rahmy TR. Differential control of growth, apoptotic activity, and gene expression in human breast cancer cells by extracts derived from medicinal herbs Zingiber officinale. J Biomed Biotechnol. 2012;2012:614356. https://doi.org/10.1155/2012/614356 [Pubmed]    [PMC Free Fulltext]   
38. Chen CY, Liu TZ, Liu YW, Tseng WC, Liu RH, Lu FJ, et al.6-shogaol (alkanone from ginger) induces apoptotic cell death of human hepatoma p53 mutant Mahlavu subline via an oxidative stress-mediated caspase-dependent mechanism. J Agric Food Chem. 2007;55(3):948-54. https://doi.org/10.1021/jf0624594 [Pubmed]   
39. Lee SH, Lee SY, Son DJ, Lee H, Yoo HS, Song S, et al. Inhibitory effect of 2'-hydroxycinnamaldehyde on nitric oxide production through inhibition of NF-kappa B activation in RAW 264.7 cells. Biochem Pharmacol. 2005;69(5):791-9. https://doi.org/10.1016/j.bcp.2004.11.013 [Pubmed]   
40. Lee JS, Jeon SM, Park EM, Huh TL, Kwon OS, Lee MK, et al. Cinnamate supplementation enhances hepatic lipid metabolism and antioxidant defense systems in high cholesterol-fed rats. J Med Food. 2003;6(3):183-91. https://doi.org/10.1089/10966200360716599 [Pubmed]   
41. Matan N, Rimkeeree H, Mawson AJ, Chompreeda P, Haruthaithanasan V, Parker M. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Int J Food Microbiol. 2006;107(2):180-5. https://doi.org/10.1016/j.ijfoodmicro.2005.07.007 [Pubmed]   
42. Khan A, Safdar M, Ali Khan MM, Khattak KN, Anderson RA. Cinnamon improves glucose and lipids of people with type 2 diabetes. Diabetes Care. 2003;26(12):3215-8. https://doi.org/10.2337/diacare.26.12.3215 [Pubmed]   
43. Kamei T, Kumano H, Iwata K, Nariai Y, Matsumoto T. The effect of a traditional Chinese prescription for a case of lung carcinoma. J Altern Complement Med. 2000;6(6):557-9. https://doi.org/10.1089/acm.2000.6.557 [Pubmed]   

This Article Cited By the following articles

Cytotoxic and cancer chemopreventive potentials of the Anthonotha macrophylla P. Beauv aqueous extract on 7,12-dimethylbenz[a]anthracene-induced breast cancer in rats
Biologia 2021; 76(2): 729.

1
 
Cytotoxic and cancer chemopreventive potentials of the Anthonotha macrophylla P. Beauv aqueous extract on 7,12-dimethylbenz[a]anthracene-induced breast cancer in rats
Biologia 2020; (): .

2
 
How to Cite this Article
Pubmed Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky. Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats. Natl J Physiol Pharm Pharmacol. 2016; 6(5): 349-358. doi:10.5455/njppp.2016.6.200220160428002


Web Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky. Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats. https://www.njppp.com/?mno=224385 [Access: December 02, 2022]. doi:10.5455/njppp.2016.6.200220160428002


AMA (American Medical Association) Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky. Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats. Natl J Physiol Pharm Pharmacol. 2016; 6(5): 349-358. doi:10.5455/njppp.2016.6.200220160428002



Vancouver/ICMJE Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky. Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats. Natl J Physiol Pharm Pharmacol. (2016), [cited December 02, 2022]; 6(5): 349-358. doi:10.5455/njppp.2016.6.200220160428002



Harvard Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky (2016) Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats. Natl J Physiol Pharm Pharmacol, 6 (5), 349-358. doi:10.5455/njppp.2016.6.200220160428002



Turabian Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky. 2016. Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (5), 349-358. doi:10.5455/njppp.2016.6.200220160428002



Chicago Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky. "Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 6 (2016), 349-358. doi:10.5455/njppp.2016.6.200220160428002



MLA (The Modern Language Association) Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky. "Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 6.5 (2016), 349-358. Print. doi:10.5455/njppp.2016.6.200220160428002



APA (American Psychological Association) Style

Mohamed Mohamed Soliman, Alshaimaa Mohammed Said Elfeky (2016) Studies on the biochemical and molecular effects of some natural herbs on experimental-induced breast cancer in Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 6 (5), 349-358. doi:10.5455/njppp.2016.6.200220160428002