E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models

Bela Paras Samariya, Gaurang B Shah.

Cited by (1)

Abstract
Background: Fructose-1,6-diphosphate (FDP), through its activity in glucose metabolism, has anticonvulsant efficacy whereas vitamin C acts as neuromodulator.

Aims and Objectives: To assess the antiepileptic effects of FDP, vitamin C and their combination in intravenous pentylenetetrazole (PTZ) and intracranial carbachol kindling model.

Materials and Methods: PTZ kindling was induced by intravenous infusion of PTZ (10 mg/ml; infusion rate: 1 ml/min) to rats. For carbachol kindling, intracranial administration of carbachol (1 μl; 5 μg/ml) was done at well-spaced intervals by introducing maximum three injections a day, and maximum nine administrations until stage 5 epileptic convulsions were produced. The treated group animals received FDP, vitamin C or both, 28 days before the kindling. During kindling, time latency to reach a seizure stage, electroencephalogram (EEG) recording, and frequency of stimulation were recorded. Brain homogenate parameters included measurement of levels of neurotransmitters, glutathione (GSH), and malondialdehyde (MDA). One-way ANOVA was used for determination of a significant difference between the groups.

Results: Slowing of seizure progression, increase in seizure threshold and required number of stimuli to reach to stage 5 convulsion was highest with FDP and Vitamin C combination followed by FDP and Vitamin C alone treatment. EEG data during the PTZ infusion indicated ictal epileptiform in disease control group and not in treatment groups. The test drugs either alone or in combination did not alter glutamate and aspartate levels in brain but increased the γ-aminobutyric acid and GSH whereas reduced MDA levels.

Conclusion: It can be concluded that both FDP and Vitamin C has beneficial effects in chemical induced kindling and their combination shows synergistic effects.

Key words: Chemical Kindling; Pentylenetetrazole; Carbachol; Electroencephalogram


 
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How to Cite this Article
Pubmed Style

Bela Paras Samariya, Gaurang B Shah. Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models. Natl J Physiol Pharm Pharmacol. 2017; 7(3): 259-264. doi:10.5455/njppp.2017.7.0927330092016


Web Style

Bela Paras Samariya, Gaurang B Shah. Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models. https://www.njppp.com/?mno=242547 [Access: January 04, 2023]. doi:10.5455/njppp.2017.7.0927330092016


AMA (American Medical Association) Style

Bela Paras Samariya, Gaurang B Shah. Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models. Natl J Physiol Pharm Pharmacol. 2017; 7(3): 259-264. doi:10.5455/njppp.2017.7.0927330092016



Vancouver/ICMJE Style

Bela Paras Samariya, Gaurang B Shah. Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models. Natl J Physiol Pharm Pharmacol. (2017), [cited January 04, 2023]; 7(3): 259-264. doi:10.5455/njppp.2017.7.0927330092016



Harvard Style

Bela Paras Samariya, Gaurang B Shah (2017) Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models. Natl J Physiol Pharm Pharmacol, 7 (3), 259-264. doi:10.5455/njppp.2017.7.0927330092016



Turabian Style

Bela Paras Samariya, Gaurang B Shah. 2017. Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models. National Journal of Physiology, Pharmacy and Pharmacology, 7 (3), 259-264. doi:10.5455/njppp.2017.7.0927330092016



Chicago Style

Bela Paras Samariya, Gaurang B Shah. "Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models." National Journal of Physiology, Pharmacy and Pharmacology 7 (2017), 259-264. doi:10.5455/njppp.2017.7.0927330092016



MLA (The Modern Language Association) Style

Bela Paras Samariya, Gaurang B Shah. "Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models." National Journal of Physiology, Pharmacy and Pharmacology 7.3 (2017), 259-264. Print. doi:10.5455/njppp.2017.7.0927330092016



APA (American Psychological Association) Style

Bela Paras Samariya, Gaurang B Shah (2017) Fructose-1,6-diphosphate and vitamin C combination increases seizure threshold in chemical kindling models. National Journal of Physiology, Pharmacy and Pharmacology, 7 (3), 259-264. doi:10.5455/njppp.2017.7.0927330092016