E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats

Shakiru Ademola Salami, Hussein Mofomosara Salahdeen, Babatunde Shuaib Anidu, Babatunde Adekunle Murtala.

Abstract
Background: Although traditional use of Tridax procumbens aqueous leaf extract (TPALE) in the management of respiratory disorders is documented, validated scientific evidence is scarce.

Aim and Objectives: Trachea smooth muscle (TSM) relaxant activity of TPALE ingestion was investigated in the presence or absence of key TSM relaxant agents. This was with the aim at elucidating relaxant activity of TPALE on TSM.

Materials and Methods: Contractile activity of TSM excised from TPALE treated (100 mg/kg) and non-treated rats was assessed pre - and post-incubation in salbutamol (10−4 M), theophylline (10−4 M), caffeine (10−4 M), naringin (10−4 M), and naringenin (10−4 M) using organ chamber connected to a force isometric transducer (Model 7004; Ugo-Basile VArese, Italy).

Results: TPALE treatment significantly inhibited contractile activity in TSM. TPALE treated rats showed significantly inhibited contractile activity of the TSM pre (45.6%) and post-incubation (35%) in theophylline when compared to control pre (90.6%) and post-incubation (60%). Incubation of TSM from control and TPALE treated rats in salbutamol, significantly inhibited contractile activity (33.2%) and (37.2%), respectively. After incubation in caffeine, TSM from TPALE treated rats showed significant inhibition in the contractile activity (30.7%) as TSM from control postincubation (38.4%). TSM of TPALE-treated group pre-incubation showed significant inhibition in contractile activity (41.8%) when compared to the TSM of TPALE-treated Group (59.3%) and control (64.5%) post-incubation in naringin. However, incubation of TSM of TPALE-treated rats in naringenin significantly inhibited contractile activity (40.4%) when compared to pre-incubation (45%) and control pre - and post-incubation, respectively (52% and 90%).

Conclusion: Calcium-activated K+ channels, β2 adrenergic stimulation, and antioxidant activity contribute to the mediation of relaxant activity by TPALE in TSM.

Key words: Trachea smooth muscle; Relaxant activity; Tridax procumbens; Theophylline; Salbutamol; Naringenin


 
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How to Cite this Article
Pubmed Style

Salami SA, Salahdeen HM, Anidu BS, Murtala BA. Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats. Natl J Physiol Pharm Pharmacol. 2022; 12(7): 1021-1026. doi:10.5455/njppp.2022.12.04064202222052022


Web Style

Salami SA, Salahdeen HM, Anidu BS, Murtala BA. Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats. https://www.njppp.com/?mno=5064 [Access: January 03, 2023]. doi:10.5455/njppp.2022.12.04064202222052022


AMA (American Medical Association) Style

Salami SA, Salahdeen HM, Anidu BS, Murtala BA. Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats. Natl J Physiol Pharm Pharmacol. 2022; 12(7): 1021-1026. doi:10.5455/njppp.2022.12.04064202222052022



Vancouver/ICMJE Style

Salami SA, Salahdeen HM, Anidu BS, Murtala BA. Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats. Natl J Physiol Pharm Pharmacol. (2022), [cited January 03, 2023]; 12(7): 1021-1026. doi:10.5455/njppp.2022.12.04064202222052022



Harvard Style

Salami, S. A., Salahdeen, . H. M., Anidu, . B. S. & Murtala, . B. A. (2022) Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats. Natl J Physiol Pharm Pharmacol, 12 (7), 1021-1026. doi:10.5455/njppp.2022.12.04064202222052022



Turabian Style

Salami, Shakiru Ademola, Hussein Mofomosara Salahdeen, Babatunde Shuaib Anidu, and Babatunde Adekunle Murtala. 2022. Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 12 (7), 1021-1026. doi:10.5455/njppp.2022.12.04064202222052022



Chicago Style

Salami, Shakiru Ademola, Hussein Mofomosara Salahdeen, Babatunde Shuaib Anidu, and Babatunde Adekunle Murtala. "Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 12 (2022), 1021-1026. doi:10.5455/njppp.2022.12.04064202222052022



MLA (The Modern Language Association) Style

Salami, Shakiru Ademola, Hussein Mofomosara Salahdeen, Babatunde Shuaib Anidu, and Babatunde Adekunle Murtala. "Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 12.7 (2022), 1021-1026. Print. doi:10.5455/njppp.2022.12.04064202222052022



APA (American Psychological Association) Style

Salami, S. A., Salahdeen, . H. M., Anidu, . B. S. & Murtala, . B. A. (2022) Roles of Ca2+ sensitive K+ channel, phosphodiesterase inhibitor, and β adrenergic activity in Tridax procumbens leaf extract relaxation of trachea smooth muscle in male Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 12 (7), 1021-1026. doi:10.5455/njppp.2022.12.04064202222052022