Hot plate method: A painful stimulus to induce anxiety in Wistar rats

Nitin S Kunnoor; Vidya K R; Basavambika A; Lohit K

doi:10.5455/njppp.2023.13.07333202211072022

2023, Vol: 13, Issue: 5

13/5Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Required files to be uploaded

Authorship Declaration

Original Research

Natl J Physiol Pharm Pharmacol. 2023; 13(3): 447-453

doi: 10.5455/njppp.2023.13.07333202211072022

Hot plate method: A painful stimulus to induce anxiety in Wistar rats

Nitin S Kunnoor, Vidya K R, Basavambika A, Lohit K.

Abstract
Background: Pain is a common stimulus that induces anxiety in both Animals and human beings.

Aim and Objective: We have undertaken this study to evaluate the induction of anxiety in Wistar rats using hot plate method.

Materials and Methods: 24 Wistar rats of either gender were used. Elevated plus maze (EPM) and light and dark arena (LDA) were used to evaluate the anxiety and hot plate analgesiometer was used to induce anxiety. After baseline reading from EPM and LDA, the Wistar rats were exposed to the hot plate and then evaluated for the induction of the anxiety behavior.

Results: After exposing to the hot plate, the ratio of time spent in the open arms to the time spent on the closed arms was decreased from 0.027 to 0.010 and also the ratio of time spent on the light chamber to the time spent on the dark chamber was decreased from 0.093 to 0.012. Hot plate method has shown statistical significant induction of anxiety as evaluated by EPM and also LDA.

Conclusion: Hot plate method is a good intervention to induce anxiety in Wistar rats. Instead of injecting drugs that causes anxiety to explore the anxiolytic effects of the drugs the hot plate analgesiometer method is a good alternative.

Key words: Anxiety; Hot Plate Method; Elevated Plus Maze; Light and Dark Arena; Painful Stimulus

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Nitin S Kunnoor Articles by Vidya K R Articles by Basavambika A Articles by Lohit K
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Kunnoor NS, R VK, A B, K L. Hot plate method: A painful stimulus to induce anxiety in Wistar rats. Natl J Physiol Pharm Pharmacol. 2023; 13(3): 447-453. doi:10.5455/njppp.2023.13.07333202211072022

Web Style

Kunnoor NS, R VK, A B, K L. Hot plate method: A painful stimulus to induce anxiety in Wistar rats. https://www.njppp.com/?mno=76981 [Access: May 03, 2023]. doi:10.5455/njppp.2023.13.07333202211072022

AMA (American Medical Association) Style

Kunnoor NS, R VK, A B, K L. Hot plate method: A painful stimulus to induce anxiety in Wistar rats. Natl J Physiol Pharm Pharmacol. 2023; 13(3): 447-453. doi:10.5455/njppp.2023.13.07333202211072022

Vancouver/ICMJE Style

Kunnoor NS, R VK, A B, K L. Hot plate method: A painful stimulus to induce anxiety in Wistar rats. Natl J Physiol Pharm Pharmacol. (2023), [cited May 03, 2023]; 13(3): 447-453. doi:10.5455/njppp.2023.13.07333202211072022

Harvard Style

Kunnoor, N. S., R, . V. K., A, . B. & K, . L. (2023) Hot plate method: A painful stimulus to induce anxiety in Wistar rats. Natl J Physiol Pharm Pharmacol, 13 (3), 447-453. doi:10.5455/njppp.2023.13.07333202211072022

Turabian Style

Kunnoor, Nitin S, Vidya K R, Basavambika A, and Lohit K. 2023. Hot plate method: A painful stimulus to induce anxiety in Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 13 (3), 447-453. doi:10.5455/njppp.2023.13.07333202211072022

Chicago Style

Kunnoor, Nitin S, Vidya K R, Basavambika A, and Lohit K. "Hot plate method: A painful stimulus to induce anxiety in Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 13 (2023), 447-453. doi:10.5455/njppp.2023.13.07333202211072022

MLA (The Modern Language Association) Style

Kunnoor, Nitin S, Vidya K R, Basavambika A, and Lohit K. "Hot plate method: A painful stimulus to induce anxiety in Wistar rats." National Journal of Physiology, Pharmacy and Pharmacology 13.3 (2023), 447-453. Print. doi:10.5455/njppp.2023.13.07333202211072022

APA (American Psychological Association) Style

Kunnoor, N. S., R, . V. K., A, . B. & K, . L. (2023) Hot plate method: A painful stimulus to induce anxiety in Wistar rats. National Journal of Physiology, Pharmacy and Pharmacology, 13 (3), 447-453. doi:10.5455/njppp.2023.13.07333202211072022

Author Login Reviewer Login About Publisher Editorial Policies Peer Review Policy Editorial & Peer Review Process Publication Ethics and Publication Malpractice Statement Conflict of Interest Policy Plagiarism Policy Protection of Research Participants (Statement On Human And Animal Rights)Corrections, Retractions & Expressions of Concern Self-Archiving Policies Statement of Informed Consent Advertising Policy Terms of Use License Information Copyright Information



Copyright © 2023 National Journal of Physiology, Pharmacy and Pharmacology All Rights Reserved. Subject to change without notice from or liability to National Journal of Physiology, Pharmacy and Pharmacology. For best results, please use Internet Explorer or Google Chrome	Contact Information Publisher publishernjppp@gmail.com Editorial query editornjppp1@gmail.com MOB - 8780040272

Contact Information

How to cite this article