E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research

Online Publishing Date:
29 / 08 / 2022

 


Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria

Kailash Jatav, Harshada Shah, Luxmi Singh, Ramanath K, Devendra Chaudhary.


Abstract
Background: India represents 3% related to the global malaria problem. Early diagnosis and treatment that are complete alongside preventive measures are modalities essential to managing the situation. Rapid diagnostic tests (RDTs) and polymerase chain reaction (PCR) that are malaria that is real-time be used to obtain an exceedingly really very early diagnosis in acutely febrile customers.

Aims and Objectives: This study aims to gauge the effectiveness of RDT bloodstream that is utilized entire from clients clinically suspected of malaria and compare it with real-time PCR.

Materials and Methods: The cross-sectional study is observationally done and made up of 158 patients admitted to Index Hospital, Indore, having a serious illness that is febrile and medical suspicion of malaria. RDT for malaria antigen and PCR that are real-time done in the bloodstream that is whole examples depending on kit guidelines.

Results: There exists a difference that is significant the nice and examples which are negative by both techniques. RT-PCR is diagnostic PCR that is real-time RDT has been good in 62 (44%) clients, whereas, real-time PCR detected the parasite in 136 (91%) customers. RDT was in reality negative for malaria antigen in 16 (12.8%) consumers, in whom RT-PCR was good. RDT failed to identify Plasmodium falciparum antigen in RT-PCR samples that can be good. RT-PCR indicates basic greater sensitiveness (82.4–95% CI 79.2–84.5%) in diagnosing malaria set alongside the quick test is an antigen. The sensitiveness of RT-PCR in detecting P. falciparum had been also high (74.2%, 95% CI 71.4–77.2). This has greater specificity than RDT in detecting P. falciparum disease (91.3%, 95% CI 89.4–95.4) in detecting P. falciparum than RDT.

Conclusion: RT-PCR has better efficacy to look for the presence of malaria parasites in acutely clients being febrile remain undiscovered by RDT. Therefore, it might be helpful for the verification of diagnoses and studies which are epidemiological.

Key words: Malaria; Rapid Diagnostic Tests; Real-Time Polymerase Chain Reaction


 
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How to Cite this Article
Pubmed Style

Jatav K, Shah H, Singh L, K R, Chaudhary D. Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria. Natl J Physiol Pharm Pharmacol. 2023; 13(4): 720-725. doi:10.5455/njppp.2023.13.08391202217082022


Web Style

Jatav K, Shah H, Singh L, K R, Chaudhary D. Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria. https://www.njppp.com/?mno=98980 [Access: March 14, 2024]. doi:10.5455/njppp.2023.13.08391202217082022


AMA (American Medical Association) Style

Jatav K, Shah H, Singh L, K R, Chaudhary D. Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria. Natl J Physiol Pharm Pharmacol. 2023; 13(4): 720-725. doi:10.5455/njppp.2023.13.08391202217082022



Vancouver/ICMJE Style

Jatav K, Shah H, Singh L, K R, Chaudhary D. Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria. Natl J Physiol Pharm Pharmacol. (2023), [cited March 14, 2024]; 13(4): 720-725. doi:10.5455/njppp.2023.13.08391202217082022



Harvard Style

Jatav, K., Shah, . H., Singh, . L., K, . R. & Chaudhary, . D. (2023) Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria. Natl J Physiol Pharm Pharmacol, 13 (4), 720-725. doi:10.5455/njppp.2023.13.08391202217082022



Turabian Style

Jatav, Kailash, Harshada Shah, Luxmi Singh, Ramanath K, and Devendra Chaudhary. 2023. Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria. National Journal of Physiology, Pharmacy and Pharmacology, 13 (4), 720-725. doi:10.5455/njppp.2023.13.08391202217082022



Chicago Style

Jatav, Kailash, Harshada Shah, Luxmi Singh, Ramanath K, and Devendra Chaudhary. "Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria." National Journal of Physiology, Pharmacy and Pharmacology 13 (2023), 720-725. doi:10.5455/njppp.2023.13.08391202217082022



MLA (The Modern Language Association) Style

Jatav, Kailash, Harshada Shah, Luxmi Singh, Ramanath K, and Devendra Chaudhary. "Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria." National Journal of Physiology, Pharmacy and Pharmacology 13.4 (2023), 720-725. Print. doi:10.5455/njppp.2023.13.08391202217082022



APA (American Psychological Association) Style

Jatav, K., Shah, . H., Singh, . L., K, . R. & Chaudhary, . D. (2023) Comparative evaluation of rapid diagnostic test and real-time PCR in clinically suspected malaria. National Journal of Physiology, Pharmacy and Pharmacology, 13 (4), 720-725. doi:10.5455/njppp.2023.13.08391202217082022