Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study

Anitha E; Neevedha K; Isswariya A; Thulasi Gokul; Priestly Vivekkumar S

doi:10.5455/njppp.2023.13.09426202214092022

2023, Vol: 13, Issue: 5

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Natl J Physiol Pharm Pharmacol. 2023; 13(5): 909-913

doi: 10.5455/njppp.2023.13.09426202214092022

Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study

Anitha E, Neevedha K, Isswariya A, Thulasi Gokul, Priestly Vivekkumar S.

Abstract
Background: Osteosarcoma is a malignant cancer that effect bone and metastasizing to many vital organs such as lungs. There are many available drugs to treat the disease including tamoxifen, methotrexate (MTX), and cisplatin which have their own side effects and hurdles to become drugs of choice for the disease. On the other hand, introduction of herbal drugs as chemotherapeutic agents opened up new arena to potentiate the existing treatment by exhibiting synergy. Piperine (PPN) is widely used drug as anti-cancer agent as well as it has anti-inflammatory, analgesic properties, and also used in the treatment of abdominal pains, tuberculosis, arthritis, and respiratory illness.

Aims and Objective: Thus, this study was designed to investigate the synergistic inhibitory potential of PPN and MTX on the MG63 osteosarcoma cell lines in vitro.

Materials and Methods: The cell lines were cultured on DMEM medium and investigated for cytotoxicity of the drugs using MTT assay at 540 nm in UV. Three groups of cell lines administered with PPN, MTX, and PPN+MTX (1:1) in various concentrations and IC50 values were calculated based on the % cell viability graphs.

Results: Results showed that the IC50 of PPN was 38.65, MTX was 123.98, and PPN+MTX was 15.13 proving the significant synergistic cytotoxic effect of PPN and MTX in inhibiting the proliferation of MG63 cell lines.

Conclusion: Further research needs to be conducted in this field to elucidate the synergistic pathways in which PPN has shown a better anti-osteosarcoma effect when combined with MTX.

Key words: Osteosarcoma; MG63 Cell Lines; Piperine; Methotrexate; Synergy; MTT Assay

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Pubmed Style

E A, K N, A I, Gokul T, S PV. Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study. Natl J Physiol Pharm Pharmacol. 2023; 13(5): 909-913. doi:10.5455/njppp.2023.13.09426202214092022

Web Style

E A, K N, A I, Gokul T, S PV. Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study. https://www.njppp.com/?mno=112208 [Access: May 01, 2023]. doi:10.5455/njppp.2023.13.09426202214092022

AMA (American Medical Association) Style

E A, K N, A I, Gokul T, S PV. Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study. Natl J Physiol Pharm Pharmacol. 2023; 13(5): 909-913. doi:10.5455/njppp.2023.13.09426202214092022

Vancouver/ICMJE Style

E A, K N, A I, Gokul T, S PV. Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study. Natl J Physiol Pharm Pharmacol. (2023), [cited May 01, 2023]; 13(5): 909-913. doi:10.5455/njppp.2023.13.09426202214092022

Harvard Style

E, A., K, . N., A, . I., Gokul, . T. & S, . P. V. (2023) Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study. Natl J Physiol Pharm Pharmacol, 13 (5), 909-913. doi:10.5455/njppp.2023.13.09426202214092022

Turabian Style

E, Anitha, Neevedha K, Isswariya A, Thulasi Gokul, and Priestly Vivekkumar S. 2023. Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study. National Journal of Physiology, Pharmacy and Pharmacology, 13 (5), 909-913. doi:10.5455/njppp.2023.13.09426202214092022

Chicago Style

E, Anitha, Neevedha K, Isswariya A, Thulasi Gokul, and Priestly Vivekkumar S. "Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study." National Journal of Physiology, Pharmacy and Pharmacology 13 (2023), 909-913. doi:10.5455/njppp.2023.13.09426202214092022

MLA (The Modern Language Association) Style

E, Anitha, Neevedha K, Isswariya A, Thulasi Gokul, and Priestly Vivekkumar S. "Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study." National Journal of Physiology, Pharmacy and Pharmacology 13.5 (2023), 909-913. Print. doi:10.5455/njppp.2023.13.09426202214092022

APA (American Psychological Association) Style

E, A., K, . N., A, . I., Gokul, . T. & S, . P. V. (2023) Synergistic effects of methotrexate and piperine on human osteosarcoma cell lines – An in-vitro study. National Journal of Physiology, Pharmacy and Pharmacology, 13 (5), 909-913. doi:10.5455/njppp.2023.13.09426202214092022

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