Abstract
Background: Seizure is the most common neurological disorder after stroke. In fact, the seizure is a chronic neurological disorder affected 1-2% of the global population. Given the side effects and toxicity of synthetic drugs, some herbal medicines are currently used in the treatment of seizure.

Aims and Objectives: The aim of this study was to evaluate the effect of Eugenol, a compound extracted from clove plant, which is effective in preventing nicotine-derived seizures in mice.

Materials and Methods: In this study, 64 mice were randomly selected. In the dose-response study, different doses of Eugenol (400, 600, and 800 mg/kg) were intraperitoneally injected to the experimental groups. The negative and positive control groups, respectively, received saline (10 ml/kg) and diazepam (0.15 mg/kg). After 30 min, the animals in all groups received intraperitoneal injections of nicotine (5 mg/kg) followed by the measurements of onset time, duration, and severity of seizures. In time-response examination, the most effective dose of Eugenol (600 mg/kg) was injected into the animals at 15, 30, 45, and 60 min before nicotine injection. Afterward, the onset, severity, and duration of seizure were determined.

Results: The results of the dose-response test showed that Eugenol doses of 400, 600, and 800 mg/kg resulted in increased time of onset, and reduced severity and duration of seizure compared to the saline group. The results of time-response revealed that the best dose was 600 mg/kg and that the best time for Eugenol injection was in 30 min before injection of nicotine.

Conclusion: The results of this study suggest that Eugenol can be effective in controlling nicotine-induces seizures.