A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations

Anil Kumar Tiwari; Sanjeev K. Singh; Ratna Pandey; Phani Bhushan Singh; Shashikant C.U. Patne; Ajay N. Gangopadhyay; Maloy B Mandal

doi:10.5455/njppp.2017.7.0623205072017

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Online Published: 15 Jul 2017

Natl J Physiol Pharm Pharmacol. 2017; 7(11): 1201-1207

doi: 10.5455/njppp.2017.7.0623205072017

A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations

Anil Kumar Tiwari, Sanjeev K. Singh, Ratna Pandey, Phani Bhushan Singh, Shashikant C.u. Patne, Ajay N. Gangopadhyay, Maloy B Mandal.

Abstract
Background: Hirschsprungs disease and other gut malformations commonly present with obstructive features of gut in pediatric age group. Problem of obstruction persists even after resection and anastomosis operation.

Aims and Objectives: This in vitro comparative study was performed to assess the gut contractility to chemical mediators such as Acetylcholine and Histamine between Hirschsprungs disease and other gut malformations.

Materials and Methods: The longitudinal muscle strips of Hirschsprungs cases and other gut malformations (non-Hirschsprungs cases) were placed in Dales organ bath containing Krebs-Ringer solution, continuously bobbled with 100% O2 at 28°C. Gut contractions were recorded using Power Lab 4/ST system and was analyzed using software CHART-5 for windows. Control contractions were recorded against initial tension of 0.5 g. Subsequently, agonist (acetylcholine, histamine)-induced contractions were recorded before and after appropriate antagonists (atropine, pheniramine). Before values of agonist-induced contractions of Hirschsprung and non-Hirschsprungs cases were compared. Values of agonistinduced contractions obtained after pretreatment with antagonists were also compared between Hirschsprung and non- Hirschsprungs cases.

Results: Acetylcholine enhanced contractions in non-Hirschsprungs cases and it caused small increase in amplitude of contractions in Hirschsprungs cases. Atropine pretreatment blocked acetylcholine-induced contractions significantly in non-Hirschsprungs cases, whereas it failed to block in the Hirschsprungs cases. Histamine augmented contractions in both Hirschsprung and non-Hirschsprungs cases. H1 antagonist, pheniramine failed to block the contractility in both the cases.

Conclusion: Findings of this study suggested that acetylcholine increased gut contractility significantly in non-Hirschsprungs cases involving muscarinic-cholinergic pathways, whereas histamine increased gut contractility in both Hirschsprungs disease and non-Hirschsprungs cases and it is not mediated by H1 receptors.

Key words: Gut Contractility; Hirschsprungs Disease; Gut Malformations; Acetylcholine, Histamine

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How to Cite this Article

Pubmed Style

Tiwari AK, Singh SK, Pandey R, Singh PB, Patne SC, Gangopadhyay AN, Mandal MB. A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations. Natl J Physiol Pharm Pharmacol. 2017; 7(11): 1201-1207. doi:10.5455/njppp.2017.7.0623205072017

Web Style

Tiwari AK, Singh SK, Pandey R, Singh PB, Patne SC, Gangopadhyay AN, Mandal MB. A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations. https://www.njppp.com/?mno=271131 [Access: June 27, 2026]. doi:10.5455/njppp.2017.7.0623205072017

AMA (American Medical Association) Style

Tiwari AK, Singh SK, Pandey R, Singh PB, Patne SC, Gangopadhyay AN, Mandal MB. A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations. Natl J Physiol Pharm Pharmacol. 2017; 7(11): 1201-1207. doi:10.5455/njppp.2017.7.0623205072017

Vancouver/ICMJE Style

Tiwari AK, Singh SK, Pandey R, Singh PB, Patne SC, Gangopadhyay AN, Mandal MB. A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations. Natl J Physiol Pharm Pharmacol. (2017), [cited June 27, 2026]; 7(11): 1201-1207. doi:10.5455/njppp.2017.7.0623205072017

Harvard Style

Tiwari, A. K., Singh, . S. K., Pandey, . R., Singh, . P. B., Patne, . S. C., Gangopadhyay, . A. N. & Mandal, . M. B. (2017) A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations. Natl J Physiol Pharm Pharmacol, 7 (11), 1201-1207. doi:10.5455/njppp.2017.7.0623205072017

Turabian Style

Tiwari, Anil Kumar, Sanjeev K. Singh, Ratna Pandey, Phani Bhushan Singh, Shashikant C.u. Patne, Ajay N. Gangopadhyay, and Maloy B Mandal. 2017. A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations. National Journal of Physiology, Pharmacy and Pharmacology, 7 (11), 1201-1207. doi:10.5455/njppp.2017.7.0623205072017

Chicago Style

Tiwari, Anil Kumar, Sanjeev K. Singh, Ratna Pandey, Phani Bhushan Singh, Shashikant C.u. Patne, Ajay N. Gangopadhyay, and Maloy B Mandal. "A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations." National Journal of Physiology, Pharmacy and Pharmacology 7 (2017), 1201-1207. doi:10.5455/njppp.2017.7.0623205072017

MLA (The Modern Language Association) Style

Tiwari, Anil Kumar, Sanjeev K. Singh, Ratna Pandey, Phani Bhushan Singh, Shashikant C.u. Patne, Ajay N. Gangopadhyay, and Maloy B Mandal. "A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations." National Journal of Physiology, Pharmacy and Pharmacology 7.11 (2017), 1201-1207. Print. doi:10.5455/njppp.2017.7.0623205072017

APA (American Psychological Association) Style

Tiwari, A. K., Singh, . S. K., Pandey, . R., Singh, . P. B., Patne, . S. C., Gangopadhyay, . A. N. & Mandal, . M. B. (2017) A comparative study of in vitro contractility between gut tissues of Hirschsprungs disease and other gut malformations. National Journal of Physiology, Pharmacy and Pharmacology, 7 (11), 1201-1207. doi:10.5455/njppp.2017.7.0623205072017

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