E-ISSN 2231-3206 | ISSN 2320-4672
 

Original Research 


Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study

Ayesha Shiraz Baig, Mohammad Shoebuddin, Muneebuddin Ahmed.

Abstract
Background: Conventional manual semen analysis is done routinely in most reproductive laboratories. Computer-assisted semen analysis (CASA), on the other hand, is more objective and timesaving counterpart to the conventional method. Different CASA software systems are available, some are fully automated. Thus, it is essential to analyze its performance and compare it with the conventional semen analysis method.

Aims and Objectives: Our objective was to analyze the same semen sample by manual method using Makler chamber and using sophisticated CASA software. Our aim was to find the level agreement between the two methods.

Materials and Methods: Ethical clearance was taken before the study. All the subjects were given proper instructions on how to collect complete semen sample. n = 60 diagnosed primary infertile male patients referred from the obstetrics and gynecology department. Semen of the same patient was analyzed by manual method and CASA software in accordance with the WHO guidelines. The results obtained were analyzed using Pearson’s correlation and Bland and Altman plot.

Results: On statistical analysis, “r” value obtained is 0.99 which is highly statistically significant. CASA and manual methods both agree with each other as proved by Bland and Altman plot.

Conclusion: Both methods can be used to screen semen samples in reproductive laboratories. Manual method is easy, feasible, requires expert analysis, and has subjective variability. CASA, on the other hand, is faster newer method with better reproducibility.

Key words: Manual Sperm Analysis; Computer-assisted Semen Analysis; Bland and Altman Plot


 
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Pubmed Style

Baig AS, Shoebuddin M, Ahmed M. Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study. Natl J Physiol Pharm Pharmacol. 2019; 9(9): 862-864. doi:10.5455/njppp.2019.9.0621817062019


Web Style

Baig AS, Shoebuddin M, Ahmed M. Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study. https://www.njppp.com/?mno=53086 [Access: December 01, 2022]. doi:10.5455/njppp.2019.9.0621817062019


AMA (American Medical Association) Style

Baig AS, Shoebuddin M, Ahmed M. Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study. Natl J Physiol Pharm Pharmacol. 2019; 9(9): 862-864. doi:10.5455/njppp.2019.9.0621817062019



Vancouver/ICMJE Style

Baig AS, Shoebuddin M, Ahmed M. Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study. Natl J Physiol Pharm Pharmacol. (2019), [cited December 01, 2022]; 9(9): 862-864. doi:10.5455/njppp.2019.9.0621817062019



Harvard Style

Baig, A. S., Shoebuddin, . M. & Ahmed, . M. (2019) Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study. Natl J Physiol Pharm Pharmacol, 9 (9), 862-864. doi:10.5455/njppp.2019.9.0621817062019



Turabian Style

Baig, Ayesha Shiraz, Mohammad Shoebuddin, and Muneebuddin Ahmed. 2019. Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study. National Journal of Physiology, Pharmacy and Pharmacology, 9 (9), 862-864. doi:10.5455/njppp.2019.9.0621817062019



Chicago Style

Baig, Ayesha Shiraz, Mohammad Shoebuddin, and Muneebuddin Ahmed. "Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study." National Journal of Physiology, Pharmacy and Pharmacology 9 (2019), 862-864. doi:10.5455/njppp.2019.9.0621817062019



MLA (The Modern Language Association) Style

Baig, Ayesha Shiraz, Mohammad Shoebuddin, and Muneebuddin Ahmed. "Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study." National Journal of Physiology, Pharmacy and Pharmacology 9.9 (2019), 862-864. Print. doi:10.5455/njppp.2019.9.0621817062019



APA (American Psychological Association) Style

Baig, A. S., Shoebuddin, . M. & Ahmed, . M. (2019) Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study. National Journal of Physiology, Pharmacy and Pharmacology, 9 (9), 862-864. doi:10.5455/njppp.2019.9.0621817062019